PAS2 Rabbit Polyclonal Antibody
Catalog# ER1902-29
PAS2 Rabbit Polyclonal Antibody
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IF-Tissue
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IHC-P
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Arabidopsis thaliana
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unconjugated
Overview
Product Name
PAS2 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within thaliana PAS2 aa 172-221 / 221.
Species Reactivity
Arabidopsis thaliana
Validated Applications
IF-Tissue, IHC-P
Molecular Weight
Predicted band size: 25 kDa
Positive Control
Arabidopsis thaliana.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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IF-Tissue
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1:50-1:100
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IHC-P
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1:50-1:200
Target
Function
Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. May be an anti-phosphatase that prevents CDKA-1 dephosphorylation and activation. Involved in the hormonal control of cell division and differentiation. Required for proliferation control of meristematic and non-meristematic cells. Negative regulator of the cell cycle.
Background References
1. Yanovsky MJ. et. al. Missense mutation in the PAS2 domain of phytochrome A impairs subnuclear localization and a subset of responses. Plant Cell. 2002 Jul;14(7):1591-603.
Sequence Similarity
Belongs to the very long-chain fatty acids dehydratase HACD family.
Tissue Specificity
High expression in young seedlings, roots, root tips, flowers and young siliques. Lower levels in leaves and stems.
Subcellular Location
Nucleus, endoplasmic reticulum membrane, cytoplasm.
Synonyms
Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase PASTICCINO 2Curated
3-hydroxyacyl-CoA dehydratase PASTICCINO 2
AtPAS2
HACD
HCD
Protein PEPINO
Protein tyrosine phosphatase-like protein
PAS2
PEP
At5g10480
ExpandImages
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Immunofluorescence staining of paraffin- embedded Arabidopsis thaliana using anti-PAS2 rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.(sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with ER1902-29 at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1/500 for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded Arabidopsis thaliana tissue using anti-PAS2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-29, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"