Beta-adaptin-like protein A Rabbit Polyclonal Antibody
Catalog# ER1902-18
Beta-adaptin-like protein A Rabbit Polyclonal Antibody
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IHC-P
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IF-Tissue
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Arabidopsis thaliana
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unconjugated
Overview
Product Name
Beta-adaptin-like protein A Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within thaliana Beta-adaptin-like protein A aa 782-831 / 841.
Species Reactivity
Arabidopsis thaliana
Validated Applications
IHC-P, IF-Tissue
Target Molecular Weight
Predicted band size: 93 kDa
Positive Control
A. thaliana tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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IHC-P
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1:50-1:200
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IF-Tissue
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1:50-1:100
Target
Function
Subunit of clathrin-associated adaptor protein complex that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules (By similarity).
Background References
1. Dortay H. et. al. Toward an interaction map of the two-component signaling pathway of Arabidopsis thaliana. J. Proteome Res. 7:3649-3660(2008)
Sequence Similarity
Belongs to the adaptor complexes large subunit family.
Subcellular Location
Golgi apparatus, trans-Golgi network, clathrin-coated vesicle membrane.
UNIPROT
Synonyms
Beta-adaptin-like protein A
At-bA-Ad
At-betaA-Ad
AP complex subunit beta-A
Adaptor protein complex AP subunit beta-A
Beta-adaptin A
Clathrin assembly protein complex beta large chain A
BETAA-AD
At5g11490
F15N18.80
Images
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Immunofluorescence staining of paraffin- embedded A. thaliana using anti-Beta-adaptin-like protein A antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.(sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes.) The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with ER1902-18 at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded A. thaliana tissue using anti-Beta-adaptin-like protein A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-18, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"