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Claudin 18.1 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1902-11

Claudin 18.1 Rabbit Polyclonal Antibody

Application

  • WB

  • FC

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

Claudin 18.1 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within human Claudin18 aa 28-80.

Species Reactivity

Human, Mouse

Validated Applications

WB, FC

Target Molecular Weight

Predicted band size: 28 kDa

Positive Control

NCI-H358 (Human lung adenocarcinoma cell) cell lysates, NCI-H1299 (Human non-small cell Lung carcinoma cell) cell lysates, F9.

Conjugation

unconjugated

RRID

AB_3069396

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • FC

  • 1:500-1:1,000

Target

Function

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is upregulated in patients with ulcerative colitis and highly overexpressed in infiltrating ductal adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated protein kinase/activator protein-1) dependent pathway regulates the expression of this gene in gastric cells. Alternatively spliced transcript variants encoding different isoforms have been identified.

Background References

1. Shi F. et. al. Claudin18 associated with corticosteroid-induced expression of surfactant proteins in pulmonary epithelial cells. J Matern Fetal Neonatal Med. 2019 Mar;32(5):809-814.

2. Baek JH. et. al. Clinical Implications of Claudin18.2 Expression in Patients With Gastric Cancer. Anticancer Res. 2019 Dec;39(12):6973-6979.

Sequence Similarity

Belongs to the claudin family.

Tissue Specificity

Isoform A1: Expression is restricted to the lung. Isoform A2: Expression is restricted to the stomach mucosa where it is predominantly observed in the epithelial cells of the pit region and the base of the gastric glands including exocrine and endocrine cells (at protein level).

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P56856 Human

SwissProt: P56857 Mouse

Synonyms

Claudin 18 antibody

Claudin-18 antibody

CLD18_HUMAN antibody

CLDN18 antibody

SFTA5 antibody

SFTPJ antibody

Images

  • Western blot analysis of Claudin 18.1 on NCI-H358 (Human lung adenocarcinoma cell) cell lysate with Rabbit anti-Claudin 18.1 antibody (<a href="/products/ER1902-11" style="font-weight: bold;text-decoration: underline;">ER1902-11</a>) at 1/5,000 dilution.<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 43 seconds; ECL: K1802<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ER1902-11" style="font-weight: bold;text-decoration: underline;">ER1902-11</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 28 kDa<br />Observed band size: 28 kDa

    Western blot analysis of Claudin 18.1 on NCI-H358 (Human lung adenocarcinoma cell) cell lysate with Rabbit anti-Claudin 18.1 antibody (ER1902-11) at 1/5,000 dilution.

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 43 seconds; ECL: K1802

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ER1902-11, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 28 kDa
    Observed band size: 28 kDa

  • Western blot analysis of Claudin 18.1 on NCI-H1299 (Human non-small cell Lung carcinoma cell) cell lysate with Rabbit anti-Claudin 18.1 antibody (<a href="/products/ER1902-11" style="font-weight: bold;text-decoration: underline;">ER1902-11</a>) at 1/5,000 dilution.<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 3 minutes; ECL: K1802<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ER1902-11" style="font-weight: bold;text-decoration: underline;">ER1902-11</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 28 kDa<br />Observed band size: 28 kDa

    Western blot analysis of Claudin 18.1 on NCI-H1299 (Human non-small cell Lung carcinoma cell) cell lysate with Rabbit anti-Claudin 18.1 antibody (ER1902-11) at 1/5,000 dilution.

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 3 minutes; ECL: K1802

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ER1902-11, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 28 kDa
    Observed band size: 28 kDa

  • Flow cytometric analysis of F9 cells labeling Claudin 18.1.<br /><br />Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (<a href="/products/ER1902-11" style="font-weight: bold;text-decoration: underline;">ER1902-11</a>, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of F9 cells labeling Claudin 18.1.

    Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (ER1902-11, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"