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EMC10 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1902-08

EMC10 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

EMC10 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within mouse EMC10 aa 1-258 / 258.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P, FC

Target Molecular Weight

Predicted band size: 27 kDa

Positive Control

SH-SY5Y cell lysates, mouse pituitary tissue, mouse testis tissue, mouse kidney tissue.

Conjugation

unconjugated

RRID

AB_3069393

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:5,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

Target

Function

Promotes angiogenesis and tissue repair in the heart after myocardial infarction. Stimulates cardiac endothelial cell migration and outgrowth via the activation of p38 MAPK, PAK and MAPK2 signaling pathways.

Background References

1. Zhou Y. et. al. EMC10 governs male fertility via maintaining sperm ion balance. J Mol Cell Biol. 2018 Dec 1;10(6):503-514.

2. Reboll MR. et. al. EMC10 (Endoplasmic Reticulum Membrane Protein Complex Subunit 10) Is a Bone Marrow-Derived Angiogenic Growth Factor Promoting Tissue Repair After Myocardial Infarction. Circulation. 2017 Nov 7;136(19):1809-1823.

Sequence Similarity

Belongs to the EMC10 family.

Tissue Specificity

Present in serum (at protein level). Increased expression seen in the left ventrice after myocardial infarction (at protein level). Expressed in the pituitary gland; very low levels in other brain regions.

Post-translational Modification

Glycosylated.

Subcellular Location

Membrane, secreted.

UNIPROT

SwissProt: Q5UCC4 Human

SwissProt: Q3TAS6 Mouse

Synonyms

Chromosome 19 open reading frame 63 antibody

emc10 antibody

EMC10_HUMAN antibody

ER membrane protein complex subunit 10 antibody

Hematopoietic signal peptide containing membrane domain containing 1 antibody

Hematopoietic signal peptide containing membrane domain containing protein 1 antibody

Hematopoietic signal peptide containing secreted 1 antibody

Hematopoietic signal peptide-containing membrane domain-containing protein 1 antibody

HSM1 antibody

HSS1 antibody

Expand

Images

  • Western blot analysis of EMC10 on different lysates with Rabbit anti-EMC10 antibody (<a href="/products/ER1902-08" style="font-weight: bold;text-decoration: underline;">ER1902-08</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate<br />Lane 2: A549 (Human lung adenocarcinoma cell) cell lysate<br />Lane 3: U-87 MG (Human glioblastoma cell) cell lysate<br />Lane 4: SH-SY5Y (Human neuroblastoma cell) cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 3 minutes; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ER1902-08" style="font-weight: bold;text-decoration: underline;">ER1902-08</a>, 1/5,000 in 5% NFDM/TBST, overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 27.3 kDa<br />Observed band size: 27 kDa

    Western blot analysis of EMC10 on different lysates with Rabbit anti-EMC10 antibody (ER1902-08) at 1/5,000 dilution.

    Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
    Lane 2: A549 (Human lung adenocarcinoma cell) cell lysate
    Lane 3: U-87 MG (Human glioblastoma cell) cell lysate
    Lane 4: SH-SY5Y (Human neuroblastoma cell) cell lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 3 minutes; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ER1902-08, 1/5,000 in 5% NFDM/TBST, overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 27.3 kDa
    Observed band size: 27 kDa

  • Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue using anti-EMC10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER1902-08" style="font-weight: bold;text-decoration: underline;">ER1902-08</a>, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue using anti-EMC10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-08, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-EMC10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER1902-08" style="font-weight: bold;text-decoration: underline;">ER1902-08</a>, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-EMC10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-08, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-EMC10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER1902-08" style="font-weight: bold;text-decoration: underline;">ER1902-08</a>, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-EMC10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-08, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of EMC10 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/ER1902-08" style="font-weight: bold;text-decoration: underline;">ER1902-08</a>, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of EMC10 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-08, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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