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SHE Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1902-03

SHE Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Tissue

  • IHC-P

Reactivity

  • Zebrafish

Conjugation

  • unconjugated

Overview

Product Name

SHE Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within zebrafish SHE aa 64-291 / 438.

Species Reactivity

Zebrafish

Validated Applications

WB, IF-Tissue, IHC-P

Target Molecular Weight

Predicted band size 48 kDa

Positive Control

Zebrafish.

Conjugation

unconjugated

RRID

AB_3069388

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

Target

Function

SH3/SH2 adaptor activity. Interacting selectively and non-covalently and simultaneously with one or more signal transduction molecules, usually acting as a scaffold to bring these molecules into close proximity either using their own SH2/SH3 domains (e.g. Grb2) or those of their target molecules (e.g. SAM68).

Background References

1. Gaudet P.et.al.Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium.Brief. Bioinformatics 12:449-462(2011).

Subcellular Location

Cytoplasm, Membrane. (Predicted)

UNIPROT

SwissProt: E7F7N7 Zebrafish

Synonyms

RP11-350G8.8 antibody

SH2 domain-containing adapter protein E antibody

She antibody

SHE_HUMAN antibody

Src homology 2 domain containing E antibody

Images

  • Immunofluorescence staining of paraffin- embedded Zebrafish using anti-SHE rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the antibody (<a href="/products/ER1902-03" style="font-weight: bold;text-decoration: underline;">ER1902-03</a>) at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor&trade; 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.

    Immunofluorescence staining of paraffin- embedded Zebrafish using anti-SHE rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the antibody (ER1902-03) at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor™ 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded Zebrafish tissue using anti-SHE antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the antibody (<a href="/products/ER1902-03" style="font-weight: bold;text-decoration: underline;">ER1902-03</a>) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded Zebrafish tissue using anti-SHE antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1902-03) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

  • Western blot analysis of SHE on zebrafish tissue lysates with Rabbit anti-SHE antibody (<a href="/products/ER1902-03" style="font-weight: bold;text-decoration: underline;">ER1902-03</a>) at 1/5,000 dilution.<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 1 minutes; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ER1902-03" style="font-weight: bold;text-decoration: underline;">ER1902-03</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 48 kDa<br />Observed band size: 48 kDa

    Western blot analysis of SHE on zebrafish tissue lysates with Rabbit anti-SHE antibody (ER1902-03) at 1/5,000 dilution.

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 1 minutes; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ER1902-03, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 48 kDa
    Observed band size: 48 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"