ZHX2 Rabbit Polyclonal Antibody
Catalog# ER1901-64
ZHX2 Rabbit Polyclonal Antibody
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WB
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IHC-P
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Human
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unconjugated
Overview
Product Name
ZHX2 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within Human ZHX2 aa 538-733 / 837.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Target Molecular Weight
92 kDa
Positive Control
Daudi cell lysates, human esophagus tissue, mouse kidney tissue, Siha.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IHC-P
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1:50-1:200
Target
Function
Zinc fingers and homeoboxes protein 2 is a protein that in humans is encoded by the ZHX2 gene. The members of the zinc fingers and homeoboxes gene family are nuclear homodimeric transcriptional repressors that interact with the A subunit of nuclear factor-Y (NF-YA) and contain two C2H2-type zinc fingers and five homeobox DNA-binding domains. This gene encodes member 2 of this gene family. In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family. Represses the promoter activity of the CDC25C gene stimulated by NFYA. May play a role in retinal development where it regulates the composition of bipolar cell populations, by promoting differentiation of bipolar OFF-type cells (By similarity). In the brain, may promote maintenance and suppress differentiation of neural progenitor cells in the developing cortex (By similarity).
Background References
1. Kawata H et al. Zinc-fingers and homeoboxes (ZHX) 2, a novel member of the ZHX family, functions as a transcriptional repressor. Biochem. J. 373:747-757 (2003).
Sequence Similarity
Belongs to the ZHX family.
Tissue Specificity
Ubiquitously expressed. Expressed in podocytes.
Subcellular Location
Nucleus.
UNIPROT
Synonyms
AFP regulator 1 antibody
AFR1 antibody
Alpha fetoprotein regulator 1 antibody
Alpha-fetoprotein regulator 1 antibody
KIAA0854 antibody
RAF antibody
Regulator of AFP antibody
Transcription factor ZHX2 antibody
ZHX2 antibody
ZHX2_HUMAN antibody
ExpandImages
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Western blot analysis of ZHX2 on Jurkat (Human T-lymphoblastic cells) lysates with Rabbit anti-ZHX2 antibody (ER1901-64) at 1/2,000 dilution.
Lysates/proteins at 15 µg/Lane.
Exposure time: 12 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ER1901-64, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 92 kDa
Observed band size: 92 kDa -
Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-ZHX2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-64, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ZHX2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-64, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Flow cytometric analysis of Zinc fingers and homeoboxes protein 2 was done on Siha cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-64, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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