FH Rabbit Polyclonal Antibody (ER1901-10)
Catalog# ER1901-10
Rabbit Polyclonal to FH
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WB
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IHC-P
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Human
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Mouse
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Rat
Lane 1: LO2 cell lysate (10 µg/Lane)
Lane 2: Mouse liver tissue lysate (20 µg/Lane)
Lane 3: Rat liver tissue lysate (20 µg/Lane)
Predicted band size: 55 kDa
Observed band size: 50 kDa
Exposure time: 1 minute;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1901-10) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
FH Rabbit Polyclonal Antibody (ER1901-10)
Immunogen
Recombinant protein within human fumarase aa 83-267.
Host
Rabbit
Positive Control
LO2 cell lysate, mouse liver tissue lysate, rat liver tissue lysate, SH-SY5Y cell lysate, mouse colon tissue lysate, rat colon tissue lysate, human liver tissue, human liver carcinoma tissue, human kidney tissue, mouse small intestine tissue, rat testis tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB:1:500-1:2,000
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IHC-P:1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 54 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
FH
SYNONYMS
FH antibody;Fumarase antibody;Fumarate hydratase antibody;Fumarate hydratase mitochondrial antibody;Fumarate hydratase, mitochondrial antibody;FUMH_HUMAN antibody;HLRCC antibody;LRCC antibody;MCL antibody;MCUL 1 antibody;MCUL1 antibody;MS709 antibody;Multiple hereditary cutaneous leiomyomata antibody
SEQUENCE SIMILARITIES
Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.
TISSUE SPECIFICITY
Expressed in red blood cells; underexpressed in red blood cells (cytoplasm) of patients with hereditary non-spherocytic hemolytic anemia of unknown etiology.
POST-TRANSLATIONAL MODIFICATION
[Isoform Cytoplasmic]: Phosphorylation at Thr-236 by PRKDC in response to DNA damage promotes translocation to the nucleus and recruitment to DNA double-strand breaks (DSBs).
SUBCELLULAR LOCATION
Mitochondrion, cytosol, nucleus, chromosome.
FUNCTION
The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.
CITATIONS
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Chen Ni,et al
Fumarate activates the IL-6/JAK/STAT3 pathway by inhibiting KDM4C-mediated H3K36me3 demethylation in FH-knockdown renal cancer cells
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IHC
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Human
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Citation
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