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Cytokeratin 7 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1803-89

Cytokeratin 7 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Cytokeratin 7 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within Human Cytokeratin 7 aa 292-431 / 469.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 51 kDa

Positive Control

HeLa cell lysate, A549 cell lysate, T-47D cell lysate, SK-Br-3 cell lysate, mouse ovary tissue lysate, mouse liver tissue lysate, rat liver tissue lysate, rat uterus tissue, human prostate cancer tissue, human breast tissue, human placenta tissue, mouse kidney tissue.

Conjugation

unconjugated

RRID

AB_3069283

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

Target

Function

Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue, where they constitute up to 85% of mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. The a-helical coiled-coil dimers associate laterally end-to-end to form 10 nm diameter filaments. Cytokeratins are useful markers of tissue differentiation and, in addition, they aid in the characterization of malignant tumors. Cytokeratin 7 (also known as sarcolectin) agglutinates normal and transformed cells with a high affinity for simple sugars. Cytokeratin 7 also inhibits the synthesis of interferon-dependent secondary proteins thus reversing the antiviral effect of interferon induction and restoring cells to their status ad primum. In normal and transformed cells, Cytokeratin 7 localizes to the membrane.

Background References

1. Petrosyan A et al. Keratin 1 plays a critical role in golgi localization of core 2 N-acetylglucosaminyltransferase M via interaction with its cytoplasmic tail. The Journal of biological chemistry 290: 6256-69 (2015).

2. Loyola AM et al. Adenoid ameloblastoma: clinicopathologic description of five cases and systematic review of the current knowledge. Oral Surg Oral Med Oral Pathol Oral Radiol 120: 368-77 (2015).

Sequence Similarity

Belongs to the intermediate filament family.

Tissue Specificity

Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.

Post-translational Modification

Arg-20 is dimethylated, probably to asymmetric dimethylarginine.

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P08729 Human

SwissProt: Q9DCV7 Mouse

SwissProt: Q6IG12 Rat

Synonyms

CK 7 antibody

CK-7 antibody

CK7 antibody

Cytokeratin 7 antibody

Cytokeratin-7 antibody

D15Wsu77e antibody

K2C7 antibody

K2C7_HUMAN antibody

K7 antibody

Keratin 7 antibody

Expand

Images

  • Western blot analysis of Cytokeratin 7 on different lysates with Rabbit anti-Cytokeratin 7 antibody (<a href="/products/ER1803-89" style="font-weight: bold;text-decoration: underline;">ER1803-89</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: A549 cell lysate<br />Lane 3: T-47D cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 51 kDa<br />Observed band size: 40-55 kDa<br /><br />Exposure time: 4 seconds; ECL: K1801;<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ER1803-89" style="font-weight: bold;text-decoration: underline;">ER1803-89</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Cytokeratin 7 on different lysates with Rabbit anti-Cytokeratin 7 antibody (ER1803-89) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: A549 cell lysate
    Lane 3: T-47D cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 51 kDa
    Observed band size: 40-55 kDa

    Exposure time: 4 seconds; ECL: K1801;

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-89) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Cytokeratin 7 on different lysates with Rabbit anti-Cytokeratin 7 antibody (<a href="/products/ER1803-89" style="font-weight: bold;text-decoration: underline;">ER1803-89</a>) at 1/1,000 dilution.<br /><br />Lane 1: mouse ovary tissue lysate (20 µg/Lane)<br />Lane 2: mouse liver tissue lysate (20 µg/Lane)<br />Lane 3: rat liver tissue lysate (20 µg/Lane)<br />Lane 4: SK-Br-3 cell lysate (10 µg/Lane)<br /><br />Exposure time: 1 minutes; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ER1803-89" style="font-weight: bold;text-decoration: underline;">ER1803-89</a>, 1/1,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 51 kDa<br />Observed band size: 40-55 kDa

    Western blot analysis of Cytokeratin 7 on different lysates with Rabbit anti-Cytokeratin 7 antibody (ER1803-89) at 1/1,000 dilution.

    Lane 1: mouse ovary tissue lysate (20 µg/Lane)
    Lane 2: mouse liver tissue lysate (20 µg/Lane)
    Lane 3: rat liver tissue lysate (20 µg/Lane)
    Lane 4: SK-Br-3 cell lysate (10 µg/Lane)

    Exposure time: 1 minutes; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ER1803-89, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 51 kDa
    Observed band size: 40-55 kDa

  • Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-Cytokeratin 7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the antibody (<a href="/products/ER1803-89" style="font-weight: bold;text-decoration: underline;">ER1803-89</a>) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-Cytokeratin 7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-89) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-Cytokeratin 7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the antibody (<a href="/products/ER1803-89" style="font-weight: bold;text-decoration: underline;">ER1803-89</a>) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-Cytokeratin 7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-89) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Cytokeratin 7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the antibody (<a href="/products/ER1803-89" style="font-weight: bold;text-decoration: underline;">ER1803-89</a>) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Cytokeratin 7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-89) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Cytokeratin 7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the antibody (<a href="/products/ER1803-89" style="font-weight: bold;text-decoration: underline;">ER1803-89</a>) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Cytokeratin 7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-89) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Cytokeratin 7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the antibody (<a href="/products/ER1803-89" style="font-weight: bold;text-decoration: underline;">ER1803-89</a>) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Cytokeratin 7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-89) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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