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SEL1L Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1803-69

SEL1L Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Tissue

  • IHC-P

Reactivity

  • Zebrafish

Conjugation

  • unconjugated

Overview

Product Name

SEL1L Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within zebrafish SEL1L aa 109-281 / 776.

Species Reactivity

Zebrafish

Validated Applications

WB, IF-Tissue, IHC-P

Molecular Weight

Predicted band size: 86 kDa

Positive Control

Zebrafish lysate, Zebrafish.

Conjugation

unconjugated

RRID

AB_3069264

Product Features

Form

Liquid

Concentration

1 mg/ml.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:200

Target

Function

Protein sel-1 homolog 1 is a protein that in humans is encoded by the SEL1L gene. May play a role in Notch signaling. May be involved in the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. In mammals, the most well-characterized ERAD machinery is the highly conserved complex of suppressor-enhancer of lin-12–like and hydroxymethylglutaryl-CoA reductase degradation protein 1 (SEL1L-HRD1), which consists of the E3 ubiquitin ligase HRD1 and its adaptor protein SEL1Lc.The Sel1L-deficient experimental model of central diabetes insipidus is unlikely to be observed in humans, as Sel1L loss is embryonically lethal. Three-week-old mice with Sel1L deficiency specifically in AVP neurons (Sel1LAVP mice) lacked AVP-positive transport vesicles in axons, a phenotype that explains the development of the central diabetes insipidus phenotype in these mice.

Background References

1. Glaeser K et al. ERAD-dependent control of the Wnt secretory factor Evi. EMBO J 37:4 (2018).

2. Kim GH et al. Hypothalamic ER-associated degradation regulates POMC maturation, feeding, and age-associated obesity. J Clin Invest 128:1125-1140 (2018).

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: Q5RIK6 Zebrafish

Synonyms

IBD2 antibody

PRO1063 antibody

Protein sel-1 homolog 1 antibody

SE1L1_HUMAN antibody

Sel 1 like protein antibody

Sel 1 suppressor of lin 12 like antibody

Sel-1L antibody

SEL1 LIKE antibody

Sel1l antibody

SEL1LIKE antibody

Expand

Images

  • Western blot analysis of SeL1L on Zebrafish lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1/500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SeL1L on Zebrafish lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1/500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  • Immunofluorescence staining of paraffin-embedded zebrafish using anti-SEL1L rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the antibody (<a href="/products/ER1803-69" style="font-weight: bold;text-decoration: underline;">ER1803-69</a>) at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor&trade; 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.

    Immunofluorescence staining of paraffin-embedded zebrafish using anti-SEL1L rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the antibody (ER1803-69) at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor™ 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-SeL1L antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the antibody (<a href="/products/ER1803-69" style="font-weight: bold;text-decoration: underline;">ER1803-69</a>) at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-SeL1L antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-69) at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"