FNDC3A Rabbit Polyclonal Antibody
Catalog# ER1803-66
FNDC3A Rabbit Polyclonal Antibody
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IF-Tissue
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IHC-P
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Zebrafish
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unconjugated
Overview
Product Name
FNDC3A Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within zebrafish FNDC3A aa 97-329 / 619.
Species Reactivity
Zebrafish
Validated Applications
IF-Tissue, IHC-P
Molecular Weight
Predicted band size: 67 kDa
Positive Control
Zebrafish.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/ml.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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IF-Tissue
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1:50-1:200
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IHC-P
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1:50-1:200
Target
Function
Fibronectin type III domain containing 7, or, FNDC7, is a human gene. Other names include: Fibronectin type III domain-containing protein 7. The encoded protein has an amino acid length of 733 and a mass of 78.2 kDa. Other isoforms may exist. This gene shares homology with other species, including: Mouse, Rat, Zebrafish, Frog, Chicken.
Background References
暂无
Subcellular Location
Golgi apparatus, Membrane.
UNIPROT
Synonyms
Fibronectin type III domain containing protein 3a antibody
FNDC3 antibody
HUGO antibody
KIAA0970 antibody
Images
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Immunofluorescence staining of paraffin- embedded zebrafish tissue using anti-FNDC3A rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the antibody (ER1803-66) at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor™ 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-FNDC3A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-66) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"