CASK Rabbit Polyclonal Antibody
Catalog# ER1803-62
CASK Rabbit Polyclonal Antibody
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WB
-
IF-Cell
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
CASK Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human CASK aa 300-500.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, FC
Target Molecular Weight
Predicted band size: 105 kDa
Positive Control
Mouse brain tissue lysate, rat brain tissue lysate, SiHa cell lysate, A549 cell lysate, SiHa,HT-29.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IF-Cell
-
1:50-1:200
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FC
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1:50-1:100
Target
Function
Multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TBR1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1.
Background References
暂无
Sequence Similarity
In the N-terminal section; belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.; Belongs to the MAGUK family.
Tissue Specificity
Ubiquitous. Expression is significantly greater in brain relative to kidney, lung, and liver and in fetal brain and kidney relative to lung and liver.
Subcellular Location
Cell membrane, Nucleus, Cytoplasm, Membrane.
Synonyms
CAGH39 antibody
Caki antibody
Calcium/calmodulin dependent serine protein kinase antibody
Calcium/calmodulin dependent serine protein kinase (MAGUK family) antibody
Calcium/calmodulin dependent serine protein kinase membrane associated guanylate kinase antibody
Calcium/calmodulin-dependent serine protein kinase antibody
CAMGUK antibody
CAMGUK protein antibody
CAMGUK, drosophila, homolog of antibody
casK antibody
ExpandImages
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Western blot analysis of CASK on different lysates with Rabbit anti-CASK antibody (ER1803-62) at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 105 kDa
Observed band size: 105 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-62) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
ICC staining CASK in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
CASK Recombinant Mouse Monoclonal Antibody [A1C5-R]
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
CASK Mouse Monoclonal Antibody [A1C5]
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
CASK Mouse Monoclonal Antibody [A1C7]
Application: WB,IHC-P,IF-Cell
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Products with the same target and pathway
CASK Recombinant Mouse Monoclonal Antibody [A1C5-R]
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
CASK Recombinant Mouse Monoclonal Antibody [A1C5-R] - BSA and Azide free
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
CASK Mouse Monoclonal Antibody [A1C5]
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated