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CTDSPL Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1803-59

CTDSPL Rabbit Polyclonal Antibody

Application

  • WB

  • FC

Reactivity

  • Human

Predicted reactivity

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

CTDSPL Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within Human CTDSPL aa 77-252 / 276.

Species Reactivity

Human (Predicted: Mouse, Rat)

Predicted species support after-sales service

Validated Applications

WB, FC

Target Molecular Weight

Predicted band size: 31 kDa

Positive Control

HepG2 cell lysate.

Conjugation

unconjugated

RRID

AB_3069254

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500

  • FC

  • 1:50-1:100

Target

Function

Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells (By similarity). Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation.

Background References

1. Yeo M et al. A novel RNA polymerase II C-terminal domain phosphatase that preferentially dephosphorylates serine 5. J Biol Chem 278:26078-26085(2003).

2. Yeo M et al. Small CTD phosphatases function in silencing neuronal gene expression. Science 307:596-600(2005).

Tissue Specificity

Expression is restricted to non-neuronal tissues.

Subcellular Location

Nucleus.

UNIPROT

SwissProt: O15194 Human

SwissProt: P58465 Mouse

Unigene: 37030 Rat

Synonyms

C3orf8 antibody

Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 3 antibody

CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like antibody

CTD small phosphatase-like protein antibody

CTDSL_HUMAN antibody

CTDSP-like antibody

Ctdspl antibody

hYA22 antibody

N-like protein antibody

NIF-like protein antibody

Expand

Images

  • Western blot analysis of CTDSPL on HepG2 cell lysate with Rabbit anti-CTDSPL antibody (<a href="/products/ER1803-59" style="font-weight: bold;text-decoration: underline;">ER1803-59</a>) at 1/500 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ER1803-59" style="font-weight: bold;text-decoration: underline;">ER1803-59</a>, 1/500 in 5% NFDM/TBST, overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/40,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 31 kDa<br />Observed band size: 31 kDa

    Western blot analysis of CTDSPL on HepG2 cell lysate with Rabbit anti-CTDSPL antibody (ER1803-59) at 1/500 dilution.

    Lysates/proteins at 20 µg/Lane.

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ER1803-59, 1/500 in 5% NFDM/TBST, overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/40,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 31 kDa
    Observed band size: 31 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"