p16INK4a Rabbit Polyclonal Antibody (ER1803-53)
Catalog# ER1803-53
Rabbit Polyclonal to CDKN2A/p16INK4a
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WB
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IF-Cell
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IHC-P
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FC
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Human
Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: Saos-2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 16 kDa
Observed band size: 16 kDa
Exposure time: 1 minute; ECL: K1801;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-53) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
p16INK4a Rabbit Polyclonal Antibody (ER1803-53)
Immunogen
Synthetic peptide within c-terminal human cdkn2a/p16ink4a.
Host
Rabbit
Positive Control
HeLa cell lysate, HEK-293 cell lysate, Saos-2 cell lysate, HeLa, human colon cancer tissue, human stomach cancer tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 16 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
CDKN2A/p16INK4a
SYNONYMS
CCM2 antibody;CDK4 inhibitor p16 INK4 antibody;CDK4I antibody;CDKN2 antibody;CDKN2A antibody;Cell cycle negative regulator beta antibody;CMM2 antibody;Cyclin dependent kinase 4 inhibitor A antibody;Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) antibody;Cyclin Dependent Kinase Inhibitor 2A antibody;Cyclin dependent kinase inhibitor 2A isoform 4 antibody;Cyclin dependent kinase inhibitor 2A isoforms 1/2/3 antibody;Cyclin dependent kinase inhibitor p16 antibody;INK4 antibody;INK4A antibody;MLM antibody;MTS1 antibody;Multiple tumor suppressor 1 antibody;p14 antibody;p16 antibody;P16INK4 antibody;p16INK4a antibody;p19 antibody;p19Arf antibody;TP16 antibody
SEQUENCE SIMILARITIES
Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.
TISSUE SPECIFICITY
Widely expressed but not detected in brain or skeletal muscle. Isoform 3 is pancreas-specific.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation seems to increase interaction with CDK4.
SUBCELLULAR LOCATION
Nucleus. Cytoplasm.
FUNCTION
This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.
CITATIONS
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Wang Rui,et al
Screening for ferroptosis genes related to endometrial carcinoma and predicting of targeted drugs based on bioinformatics
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IHC
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Human
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Citation
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