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TMEM248 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1803-48

TMEM248 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • FC

Reactivity

  • Human

  • Rat

Predicted reactivity

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

TMEM248 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within Human TMEM248 aa 265-314 / 314.

Species Reactivity

Human, Rat (Predicted: Mouse)

Predicted species support after-sales service

Validated Applications

WB, IF-Cell, FC

Target Molecular Weight

Predicted band size: 35 kDa

Positive Control

K-562 cell lysate, SK-Br-3 cell lysate, A-172 cell lysate, HeLa cell lysate, PC-12 cell lysate, HUVEC, SH-SY-5Y, SiHa.

Conjugation

unconjugated

RRID

AB_3069244

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

  • IF-Cell

  • 1:50-1:200

  • FC

  • 1:50-1:100

Target

Function

Transmembrane protein 248 is a protein that is encoded by the TMEM248 gene. TMEM248, also known as C7orf42, is a gene that is found in humans and encodes a transmembrane protein with a function that is not yet well understood by the scientific community. This gene contains four transmembrane domains, and is composed of six exons. The protein TMEM248 encodes is 314 amino acid long protein containing four transmembrane regions. The theoretical molecular weight of the 314 amino acid TMEM248 protein is approximately 35kDa and the theoretical pI is approximately 6. TMEM248 is expected to have a subcellular localization in the plasma membrane and vesicles. Based on predictions from Chou and Fasman Secondary Structure Prediction Server, TMEM248 is primarily made up of beta sheets and helices. TMEM248 protein has four transmembrane domains. TMEM248 (transmembrane protein 248), or C7orf42 (chromosome 7, open reading frame 42) is predicted to be an integral component of the plasma membrane. It has low tissue specificity, meaning it is expressed in most tissues throughout the human body. TMEM248 has cytoplasmic expression in multiple different tissue types, but is the most abundant in the gastrointestinal tract. TMEM248 may play a role in cancer development. It was shown to be more highly expressed in cases of colon, breast, lung, ovarian, brain, and renal cancers.

Background References

暂无

Sequence Similarity

Belongs to the TMEM248 family.

Subcellular Location

Membrane.

UNIPROT

SwissProt: Q9NWD8 Human

SwissProt: Q3TBN1 Mouse

SwissProt: Q6AY76 Rat

Synonyms

Transmembrane protein 248

TMEM248

C7orf42

Images

  • Western blot analysis of TMEM248 on different lysates with Rabbit anti-TMEM248 antibody (<a href="/products/ER1803-48" style="font-weight: bold;text-decoration: underline;">ER1803-48</a>) at 1/1,000 dilution.<br /><br />Lane 1: K-562 cell lysate<br />Lane 2: SK-Br-3 cell lysate<br />Lane 3: A-172 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 35 kDa<br />Observed band size: 30 kDa<br /><br />Exposure time: 1 minute 16 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ER1803-48" style="font-weight: bold;text-decoration: underline;">ER1803-48</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TMEM248 on different lysates with Rabbit anti-TMEM248 antibody (ER1803-48) at 1/1,000 dilution.

    Lane 1: K-562 cell lysate
    Lane 2: SK-Br-3 cell lysate
    Lane 3: A-172 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 35 kDa
    Observed band size: 30 kDa

    Exposure time: 1 minute 16 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-48) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of TMEM248 on different lysates with Rabbit anti-TMEM248 antibody (<a href="/products/ER1803-48" style="font-weight: bold;text-decoration: underline;">ER1803-48</a>) at 1/5,000 dilution.<br /><br />Lane 1: K-562 cell lysate (no heat)<br />Lane 2: HeLa cell lysate (hot lysis)<br />Lane 3: PC-12 cell lysate<br /><br />Notice: no heat means the lysate is not boiled.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 35 kDa<br />Observed band size: 30 kDa<br /><br />Exposure time: 1 minute 16 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ER1803-48" style="font-weight: bold;text-decoration: underline;">ER1803-48</a>) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TMEM248 on different lysates with Rabbit anti-TMEM248 antibody (ER1803-48) at 1/5,000 dilution.

    Lane 1: K-562 cell lysate (no heat)
    Lane 2: HeLa cell lysate (hot lysis)
    Lane 3: PC-12 cell lysate

    Notice: no heat means the lysate is not boiled.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 35 kDa
    Observed band size: 30 kDa

    Exposure time: 1 minute 16 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-48) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • ICC staining Tmem248 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Tmem248 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining Tmem248 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Tmem248 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining Tmem248 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Tmem248 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"