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APR3 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1803-44

APR3 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

APR3 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within mouse APR3 aa 174-223 / 223.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P, FC

Target Molecular Weight

Predicted band size: 25 kDa

Positive Control

Human skin tissue lysates, A549, SH-SY-5Y, mouse kidney tissue, MCF-7.

Conjugation

unconjugated

RRID

AB_3069240

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-2,000

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:500

  • FC

  • 1:50-1:100

Target

Function

This gene is thought to be involved in apoptosis, and may also be involved in hematopoietic development and differentiation. The use of alternative splice sites and promotors result in multiple transcript variants encoding different isoforms. Promotes osteoblast cell differentiation and terminal mineralization. Plays a role in inducing the cell cycle arrest via inhibiting CCND1 expression in all-trans-retinoic acid (ATRA) signal pathway.

Background References

1. Zou X et al. NELL-1 binds to APR3 affecting human osteoblast proliferation and differentiation. FEBS Lett. 585:2410-2418 (2011).

2. Yu F et al. Apoptosis related protein 3, an ATRA-upregulated membrane protein arrests the cell cycle at G1/S phase by decreasing the expression of cyclin D1. Biochem. Biophys. Res. Commun. 358:1041-1046 (2007).

Tissue Specificity

Weakly expressed in hematopoietic cell lines.

Subcellular Location

Nucleus envelope, Cell membrane.

UNIPROT

SwissProt: Q6UW56 Human

SwissProt: Q6PGD0 Mouse

SwissProt: B2RYU3 Rat

Synonyms

APR-3

ATRAID

APR3

C2orf28

HSPC013

UNQ214/PRO240

Images

  • Western blot analysis of APR3 on human skin tissue lysates using anti-APR3 antibody.<br /> Lane 1: Anti-APR3 antibody (1/500).<br /> Lane 2: Anti-APR3 antibody, pre-incubated with the immunizaiton peptide.<br />

    Western blot analysis of APR3 on human skin tissue lysates using anti-APR3 antibody.
    Lane 1: Anti-APR3 antibody (1/500).
    Lane 2: Anti-APR3 antibody, pre-incubated with the immunizaiton peptide.

  • ICC staining APR3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining APR3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining APR3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining APR3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-APR3 antibody (<a href="/products/ER1803-44" style="font-weight: bold;text-decoration: underline;">ER1803-44</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER1803-44" style="font-weight: bold;text-decoration: underline;">ER1803-44</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-APR3 antibody (ER1803-44) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-44) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"