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CACNB1 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1803-29

CACNB1 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

CACNB1 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within mouse CACNB1 aa 439-488 / 597.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Cell

Target Molecular Weight

Predicted band size: 65/53 kDa

Positive Control

rat heart tissue, human tonsil tissue, human kidney tissue, human fetal skeletal muscle tissue, mouse brain tissue, rat skeletal muscle tissue.

Conjugation

unconjugated

RRID

AB_3073442

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

  • IF-Cell

  • 1:50

Target

Function

The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting.

Background References

1. Brust P F et al. Human neuronal voltage-dependent calcium channels: studies on subunit structure and role in channel assembly. Neuropharmacology 32:1089-1102 (1993).

2. Sjoeblom T et al. The consensus coding sequences of human breast and colorectal cancers. Science 314:268-274 (2006).

Sequence Similarity

Belongs to the calcium channel beta subunit family.

Tissue Specificity

Detected in heart ventricle (at protein level). Isoform 1 and isoform 3 are expressed in brain, heart, spleen, central nervous system and neuroblastoma cells. Isoform 2 is expressed in skeletal muscle.

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: Q02641 Human

SwissProt: Q8R3Z5 Mouse

SwissProt: P54283 Rat

Synonyms

CAB1 antibody

CACB1_HUMAN antibody

CACNB1 antibody

CACNLB1 antibody

Calcium channel L type beta 1 polypeptide antibody

Calcium channel voltage dependent beta 1 subunit antibody

Calcium channel voltage-dependent subunit beta 1 antibody

CCHLB1 antibody

Dihydropyridine sensitive L type calcium channel beta 1 subunit antibody

MGC41896 antibody

Expand

Images

  • Western blot analysis of CACNB1 on different lysates with Rabbit anti-CACNB1 antibody (<a href="/products/ER1803-29" style="font-weight: bold;text-decoration: underline;">ER1803-29</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br />Lane 2: Rat brain tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 3 minutes; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ER1803-29" style="font-weight: bold;text-decoration: underline;">ER1803-29</a>, 1/2,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 65 kDa<br />Observed band size: 53 kDa

    Western blot analysis of CACNB1 on different lysates with Rabbit anti-CACNB1 antibody (ER1803-29) at 1/2,000 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: Rat brain tissue lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 3 minutes; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ER1803-29, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 65 kDa
    Observed band size: 53 kDa

  • Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-CACNB1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-CACNB1 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-CACNB1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-CACNB1 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-CACNB1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-CACNB1 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CACNB1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CACNB1 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-CACNB1 antibody (<a href="/products/ER1803-29" style="font-weight: bold;text-decoration: underline;">ER1803-29</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER1803-29" style="font-weight: bold;text-decoration: underline;">ER1803-29</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-CACNB1 antibody (ER1803-29) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-29) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"