CACNB3 Rabbit Polyclonal Antibody
Catalog# ER1803-24
CACNB3 Rabbit Polyclonal Antibody
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WB
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IHC-P
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
CACNB3 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within Human CACNB3 aa 435-484 / 484.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, FC
Target Molecular Weight
Predicted band size: 55 kDa
Positive Control
Rat brain tissue lysate, human uterus tissue, mouse ovary tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:5000
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
Target
Function
Regulatory subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents. Increases CACNA1B peak calcium current and shifts the voltage dependencies of channel activation and inactivation. Increases CACNA1C peak calcium current and shifts the voltage dependencies of channel activation and inactivation.
Background References
1. Collin T et al. Cloning, chromosomal location and functional expression of the human voltage-dependent calcium-channel beta 3 subunit. Eur J Biochem 220:257-262 (1994).
2. Yamada Y et al. The structures of the human calcium channel alpha 1 subunit (CACNL1A2) and beta subunit (CACNLB3) genes. Genomics 27:312-319 (1995).
Sequence Similarity
Belongs to the calcium channel beta subunit family.
Tissue Specificity
Expressed mostly in brain, colon and ovary.
Subcellular Location
Cytoplasm.
Synonyms
CAB3 antibody
CACB3_HUMAN antibody
Cacnb3 antibody
CACNLB3 antibody
Calcium channel voltage dependent beta 3 subunit antibody
Calcium channel voltage-dependent subunit beta 3 antibody
FLJ58949 antibody
Voltage-dependent L-type calcium channel subunit beta-3 antibody
Images
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Western blot analysis of CACNB3 on Rat brain tissue lysate with Rabbit anti-CACNB3 antibody (ER1803-24) at 1/5,000 dilution.
Lysates/proteins at 30 µg/Lane.
Exposure time: 12 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ER1803-24, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature -
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CACNB3 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-CACNB3 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse ovary tissue using anti-CACNB3 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"