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NDUFS3 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1803-14

NDUFS3 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

NDUFS3 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within Human NDUFS3 aa 7-239 / 264.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, FC

Target Molecular Weight

Predicted band size: 30 kDa

Positive Control

LOVO, MCF-7, rat epididymis tissue, human liver tissue, mouse brain tissue.

Conjugation

unconjugated

RRID

AB_3069213

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:2,000

  • FC

  • 1:50-1:100

Target

Function

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Background References

1. Xu G et al. Global profiling of protease cleavage sites by chemoselective labeling of protein N-termini. Proc Natl Acad Sci USA 106:19310-19315 (2009).

2. Murray J et al. The subunit composition of the human NADH dehydrogenase obtained by rapid one-step immunopurification. J Biol Chem 278:13619-13622 (2003).

Sequence Similarity

Belongs to the complex I 30 kDa subunit family.

Subcellular Location

Mitochondrion inner membrane.

UNIPROT

SwissProt: O75489 Human

SwissProt: Q9DCT2 Mouse

SwissProt: D3ZG43 Rat

Synonyms

CI 30 antibody

CI 30KD antibody

CI-30kD antibody

Complex I 30KD antibody

Complex I 30kDa subunit antibody

COMPLEX I, MITOCHONDRIAL RESPIRATORY CHAIN, 30-KD SUBUNIT antibody

Complex I-30kD antibody

mitochondrial antibody

NADH coenzyme Q reductase antibody

NADH dehydrogenase (ubiquinone) Fe S protein 3 30kDa antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of NDUFS3 on different lysates with Rabbit anti-NDUFS3 antibody (<a href="/products/ER1803-14" style="font-weight: bold;text-decoration: underline;">ER1803-14</a>) at 1/1,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-NDUFS3 KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 30 kDa<br />Observed band size: 30 kDa<br /><br />Exposure time: 9 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ER1803-14" style="font-weight: bold;text-decoration: underline;">ER1803-14</a>) at 1/1,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of NDUFS3 on different lysates with Rabbit anti-NDUFS3 antibody (ER1803-14) at 1/1,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-NDUFS3 KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 30 kDa
    Observed band size: 30 kDa

    Exposure time: 9 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-14) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-NDUFS3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-NDUFS3 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-NDUFS3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-NDUFS3 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NDUFS3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NDUFS3 antibody. Counter stained with hematoxylin.

  • Western blot analysis of NDUFS3 on different lysates with Rabbit anti-NDUFS3 antibody (<a href="/products/ER1803-14" style="font-weight: bold;text-decoration: underline;">ER1803-14</a>) at 1/5,000 dilution.<br /><br />Lane 1:HepG2 (Human hepatocellular carcinoma cell) cell lysate <br />Lane 2: Mouse herat tissue lysate<br />Lane 3: Rat heart tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 8 seconds; ECL: K1801<br /><br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ER1803-14" style="font-weight: bold;text-decoration: underline;">ER1803-14</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 30 kDa<br />Observed band size: 30 kDa

    Western blot analysis of NDUFS3 on different lysates with Rabbit anti-NDUFS3 antibody (ER1803-14) at 1/5,000 dilution.

    Lane 1:HepG2 (Human hepatocellular carcinoma cell) cell lysate
    Lane 2: Mouse herat tissue lysate
    Lane 3: Rat heart tissue lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 8 seconds; ECL: K1801


    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ER1803-14, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 30 kDa
    Observed band size: 30 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Alternative Products

metafield image

NDUFS3 Recombinant Rabbit Monoclonal Antibody [JU10-32]

Application: WB,IHC-P,IF-Cell,IF-Tissue,IP

Reactivity: Human

Conjugate: unconjugated

Products with the same target and pathway

metafield image

NDUFS3 Recombinant Rabbit Monoclonal Antibody [JU10-32]

Application: WB,IHC-P,IF-Cell,IF-Tissue,IP

Reactivity: Human

Conjugate: unconjugated