Orai1 Rabbit Polyclonal Antibody (ER1803-11)
Catalog# ER1803-11
Rabbit Polyclonal to Orai1
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WB
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IHC-P
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Human
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Mouse
Lane 1: HeLa cell lysate
Lane 2: C2C12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 33 kDa
Observed band size: 50 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-11) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
Orai1 Rabbit Polyclonal Antibody (ER1803-11)
Immunogen
Synthetic peptide within mouse orai1 aa 181-230 / 304.
Host
Rabbit
Positive Control
human skin tissue, human esophagus tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IHC-P
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1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 33 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Orai1
SYNONYMS
Calcium release activated calcium channel protein 1 antibody;Calcium release activated calcium modulator 1 antibody;Calcium release-activated calcium channel protein 1 antibody;CRACM 1 antibody;CRACM1 antibody;CRCM1 antibody;CRCM1_HUMAN antibody;FLJ14466 antibody;Orai 1 antibody;ORAI calcium release activated calcium modulator 1 antibody;orai1 antibody;ORAT1 antibody;Protein orai 1 antibody;Protein orai-1 antibody;TMEM 142A antibody;TMEM142A antibody;Transmembrane protein 142A antibody
SEQUENCE SIMILARITIES
Belongs to the Orai family.
TISSUE SPECIFICITY
Expressed in naive CD4 and CD8 T cells (at protein level). Expressed at similar levels in naive and effector T helper cells.
POST-TRANSLATIONAL MODIFICATION
N-glycosylated. N-glycosylation inhibits channel activity in T cells.; Ubiquitinated.; Cys-195 is oxidated, leading to inactivation of channel activity.
SUBCELLULAR LOCATION
Cell membrane.
FUNCTION
Orai1 (ORAI calcium release-activated calcium modulator 1), also known as ORAT1, CRACM1 (calcium release-activated calcium modulator 1) or TMEM142A (transmembrane protein 142A), is a 301 amino acid multi-pass membrane protein that belongs to the Orai family of proteins. Localizing to the plasma membrane, Orai1 plays an important role in store-operated calcium (SOC) entry, a process involving Ca2+ influx and replenishment of Ca2+ stores formerly emptied through the action of inositol 1,4,5-trisphosphate production and other Ca2+ mobilizing agents. Specifically, Orai1 functions as a pore subunit of the store-operated calcium release-activated calcium channel (CRAC) and is essential for proper function of the CRAC channel. CRAC channels are responsible for mediating calcium influx in T-cells and play an important role in the immune response. Mutations in the gene encoding Orai1 can result in severe combined immunodeficiency (SCID).
CITATIONS
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Zeng, Z., Cheng, M., Li, M., Wang, T., Wen, F., Sanderson, M. J., Sneyd, J., Shen, Y., & Chen, J.
Inherent differences of small airway contraction and Ca2+ oscillations in airway smooth muscle cells between BALB/c and C57BL/6 mouse strains
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IF
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Mouse
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Citation
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