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ERGI3 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1802-98

ERGI3 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

ERGI3 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within Human ERGI3 aa 334-383 / 383.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P, FC

Target Molecular Weight

Predicted band size: 54 kDa

Positive Control

Caco-2 (Human colorectal adenocarcinoma cell) cell lysate, A549 (Human lung adenocarcinoma cell) cell lysate, mouse placenta tissue lysate, mouse bone marrow tissue lysate, rat placenta tissue lysate, rat bone marrow tissue lysate, LOVO, SiHa, human colon cancer tissue, human placenta tissue, human uterus tissue, mouse brain tissue.

Conjugation

unconjugated

RRID

AB_3069198

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

Target

Function

Belongs to the ERGIC family. The ERGIC lies between the rough endoplasmic reticulum and Golgi on the secretory pathway. The vesicular-tubular cluster (VTC), also referred to as the ER-Golgi intermediate compartment (or ERGIC), is an organelle in eukaryotic cells. This compartment mediates trafficking between the endoplasmic reticulum and Golgi complex, facilitating the sorting of cargo. ERGI3 possible role in transport between endoplasmic reticulum and Golgi.

Background References

暂无

Sequence Similarity

Belongs to the ERGIC family.

Subcellular Location

Endoplasmic reticulum. Golgi apparatus.

UNIPROT

SwissProt: Q9Y282 Human

SwissProt: Q9CQE7 Mouse

Synonyms

2310015B14Rik antibody

AV318804 antibody

C20orf47 antibody

CGI 54 antibody

D2Ucla1 antibody

dJ477O4.2 antibody

DKFZp547A2190 antibody

Endoplasmic reticulum Golgi intermediate compartment protein 3 antibody

endoplasmic reticulum localized protein ERp43 antibody

Endoplasmic reticulum-Golgi intermediate compartment protein 3 antibody

Expand

Images

  • Western blot analysis of ERGI3 on different lysates with Rabbit anti-ERGI3 antibody (<a href="/products/ER1802-98" style="font-weight: bold;text-decoration: underline;">ER1802-98</a>) at 1/5,000 dilution.<br /><br />Lane 1: Caco-2 (Human colorectal adenocarcinoma cell) cell lysate<br />Lane 2: A549 (Human lung adenocarcinoma cell) cell lysate<br />Lane 3: Mouse placenta tissue lysate<br />Lane 4: Mouse bone marrow tissue lysate<br />Lane 5: Rat placenta tissue lysate<br />Lane 6: Rat bone marrow tissue lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 10 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ER1802-98" style="font-weight: bold;text-decoration: underline;">ER1802-98</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 43 kDa<br />Observed band size: 54 kDa

    Western blot analysis of ERGI3 on different lysates with Rabbit anti-ERGI3 antibody (ER1802-98) at 1/5,000 dilution.

    Lane 1: Caco-2 (Human colorectal adenocarcinoma cell) cell lysate
    Lane 2: A549 (Human lung adenocarcinoma cell) cell lysate
    Lane 3: Mouse placenta tissue lysate
    Lane 4: Mouse bone marrow tissue lysate
    Lane 5: Rat placenta tissue lysate
    Lane 6: Rat bone marrow tissue lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 10 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ER1802-98, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 43 kDa
    Observed band size: 54 kDa

  • ICC staining ERGI3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining ERGI3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining ERGI3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining ERGI3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ERGI3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ERGI3 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ERGI3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ERGI3 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-ERGI3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-ERGI3 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ERGI3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ERGI3 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"