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sfGFP Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1802-92

sfGFP Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Species independent

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

sfGFP Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide immune sequence is RDHMVLHEYVNAAGITC.

Species Reactivity

Species independent

Validated Applications

WB

Positive Control

GFP11-tagged fusion protein.

Conjugation

unconjugated

RRID

AB_3069192

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Tagging proteins with a functional sequence is a powerful way to access function, and inserting tags at endogenous genomic loci allows the preservation of a near-native cellular background. GFP11 is the 11th beta-strand of the superfolder GFP beta-barrel structure. When expressed in the same cell, GFP11 and its complementary GFP fragment (GFP1-10) enable functional GFP tagging upon complementation. The key advantage of the GFP11 is its small size (16 aa): this allows commercial ssDNA oligomers to be used as HDR donors, circumventing any requirement for molecular cloning.

Background References

1. Leonetti MD et al. A scalable strategy for high-throughput GFP tagging of endogenous human proteins. Proc Natl Acad Sci USA 113(25):E3501-8 (2016).

Images

  • Western blot analysis of sfGFP on GFP11-tagged recombinant protein lysate with Rabbit anti-sfGFP antibody (<a href="/products/ER1802-92" style="font-weight: bold;text-decoration: underline;">ER1802-92</a>) at 1/5,000 dilution.<br /><br />Lysates/proteins at 10 µg/Lane.<br />Exposure time: 20 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ER1802-92" style="font-weight: bold;text-decoration: underline;">ER1802-92</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 30 kDa<br />Observed band size: 30 kDa

    Western blot analysis of sfGFP on GFP11-tagged recombinant protein lysate with Rabbit anti-sfGFP antibody (ER1802-92) at 1/5,000 dilution.

    Lysates/proteins at 10 µg/Lane.
    Exposure time: 20 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ER1802-92, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 30 kDa
    Observed band size: 30 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation