GOT1 Rabbit Polyclonal Antibody
Catalog# ER1802-48
GOT1 Rabbit Polyclonal Antibody
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WB
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IHC-P
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FC
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Human
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unconjugated
Overview
Product Name
GOT1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within N-terminal Human GOT1.
Species Reactivity
Human
Validated Applications
WB, IHC-P, FC
Molecular Weight
Predicted band size: 46 kDa
Positive Control
K562, human liver tissue, human colon tissue, human heart tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
Target
Function
Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
Background References
1. D\'Aniello A et al. Amino acids and transaminases activity in ventricular CSF and in brain of normal and Alzheimer patients. Neurosci Lett 388:49-53 (2005).
2. Zlotnik A et al. Relationship between glutamate, GOT and GPT levels in maternal and fetal blood: a potential mechanism for fetal neuroprotection. Early Hum Dev 88:773-778 (2012).
Sequence Similarity
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
Subcellular Location
Cytoplasm.
UNIPROT
Synonyms
CGI-141 antibody
GCT2 antibody
Germ cell tumor 2 antibody
Golgi transport 1 homolog B antibody
Golgi transport 1B antibody
GOLT1B antibody
GOT1 antibody
GOT1B_HUMAN antibody
hGOT1a antibody
Putative NF-kappa-B-activating protein 470 antibody
ExpandImages
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☑ Knockdown (KD)
Western blot analysis of GOT1 on different lysates with Rabbit anti-GOT1 antibody (ER1802-48) at 1/1,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-GOT1 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1802-48) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of GOT1 on K562 cell lysate using anti-GOT1 antibody at 1/500 dilution.
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Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GOT1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-GOT1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-GOT1 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"