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Interferon alpha Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1802-4

Interferon alpha Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Interferon alpha Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within mouse IFNA1 aa 140-181.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P, FC

Target Molecular Weight

Predicted band size: 22 kDa

Positive Control

N2A, SHG-44, SH-SY5Y, PC-3M, rat brain tissue, human kidney tissue, mouse brain tissue.

Conjugation

unconjugated

RRID

AB_3069138

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

Target

Function

The genes encoding type I interferons (IFNs), which include 14 IFN-α genes (two of which are IFN-α1 and IFN-α13), one IFN-β gene, one IFN-w (also known as IFN-α II1) gene and a number of IFN-w pseudogenes, are clustered on human chromosome 9. IFN-α and -β are cytokines that are widely known to induce potent antiviral activity. They exert a variety of other biological effects, including antitumor and immunomodulatory activities and are increasingly used clinically to treat a range of malignancies, myelodysplasias and autoimmune diseases. IFN-w is antigenically different from human IFN-α, IFN-β or IFN-γ, but is a component of natural mixtures of IFN species produced by virus-induced leukocytes or Burkitt's lymphoma cells. The type I interferon receptor (IFN-αR) interacts with IFN-α, IFN-β and IFN-w, and seems to be a multisubunit receptor.

Background References

暂无

Sequence Similarity

Belongs to the alpha/beta interferon family.

Subcellular Location

Secreted.

UNIPROT

SwissProt: P01562 Human

SwissProt: P01572 Mouse

SwissProt: P05011 Rat

Synonyms

IFL antibody

IFN alpha antibody

IFN alpha 1/13 antibody

IFN alpha 1b antibody

IFN alphaD antibody

IFN antibody

IFN-alpha-1/13 antibody

IFNA@ antibody

IFNA1 antibody

IFNA1_HUMAN antibody

Expand

Images

  • Western blot analysis of Interferon alpha on mouse IFNA1 recombinant protein with Rabbit anti-Interferon alpha antibody (<a href="/products/ER1802-4" style="font-weight: bold;text-decoration: underline;">ER1802-4</a>) at 1/5,000 dilution.<br /><br />Lysates/proteins at 50 ng/Lane.<br /><br />Exposure time: 3 minutes; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ER1802-4" style="font-weight: bold;text-decoration: underline;">ER1802-4</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Interferon alpha on mouse IFNA1 recombinant protein with Rabbit anti-Interferon alpha antibody (ER1802-4) at 1/5,000 dilution.

    Lysates/proteins at 50 ng/Lane.

    Exposure time: 3 minutes; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1802-4) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of PC-3M cells labeling Interferon alpha with Rabbit anti-Interferon alpha antibody (<a href="/products/ER1802-4" style="font-weight: bold;text-decoration: underline;">ER1802-4</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Interferon alpha antibody (<a href="/products/ER1802-4" style="font-weight: bold;text-decoration: underline;">ER1802-4</a>) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.<br /><br />β-tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 647, <a href="/products/HA1127" style="font-weight: bold;text-decoration: underline;">HA1127</a>) were used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of PC-3M cells labeling Interferon alpha with Rabbit anti-Interferon alpha antibody (ER1802-4) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Interferon alpha antibody (ER1802-4) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    β-tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution.

  • ICC staining IFNA1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining IFNA1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining IFNA1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining IFNA1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining IFNA1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining IFNA1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-IFNA1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-IFNA1 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-IFNA1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-IFNA1 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue tissue using anti-IFNA1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue tissue using anti-IFNA1 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"