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MSI2 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1706-59

MSI2 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

MSI2 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within Human MSI2 aa 1-185 / 328.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P, FC

Target Molecular Weight

Predicted band size: 35/37 kDa

Positive Control

K562, HepG2, PC-12, SH-SY5Y, rat epididymis tissue, human breast tissue, human placenta tissue, mouse brain tissue.

Conjugation

unconjugated

RRID

AB_3069074

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:50-1:4,000

  • FC

  • 1:50-1:100

Target

Function

Msi2 (musashi homolog 2), also known as MSI2H, is a 328 amino acid protein that localizes to the cytoplasm and contains two RRM (RNA recognition motif) domains. Expressed ubiquitously at low levels, Msi2 functions as an RNA binding protein that, by regulating the expression of target mRNAs, is thought to play a role in the proliferation and maintenance of stem cells within the central nervous system. Msi2 is subject to post-translational phosphorylation and is upregulated in response to brain injury, suggesting a role in healing and brain tissue regeneration. Chromosomal aberrations involving the Msi2 gene are associated with the progression of chronic myeloid leukemia. Multiple isoforms of Msi2 exist due to alternative splicing events.

Background References

暂无

Sequence Similarity

Belongs to the Musashi family.

Tissue Specificity

Ubiquitous; detected at low levels.

Post-translational Modification

Phosphorylated.

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: Q96DH6 Human

SwissProt: Q920Q6 Mouse

SwissProt: F1LWE6 Rat

Synonyms

FLJ36569 antibody

MGC3245 antibody

Msi2 antibody

MSI2/HOXA9 fusion gene, included antibody

MSI2H antibody

MSI2H_HUMAN antibody

Musashi 2 antibody

Musashi homolog 2 antibody

Musashi RNA binding protein 2 antibody

Musashi, Drosophila, homolog of, 2 antibody

Expand

Images

  • Western blot analysis of MSI2 on different lysates with Rabbit anti-MSI2 antibody (<a href="/products/ER1706-59" style="font-weight: bold;text-decoration: underline;">ER1706-59</a>) at 1/2,000 dilution.<br /><br />Lane 1: K562 cell lysate<br />Lane 2: PC-12 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 35/37 kDa<br />Observed band size: 35/37 kDa<br /><br />Exposure time: 2 minutes;<br /><br />12% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ER1706-59" style="font-weight: bold;text-decoration: underline;">ER1706-59</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of MSI2 on different lysates with Rabbit anti-MSI2 antibody (ER1706-59) at 1/2,000 dilution.

    Lane 1: K562 cell lysate
    Lane 2: PC-12 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 35/37 kDa
    Observed band size: 35/37 kDa

    Exposure time: 2 minutes;

    12% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-59) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of MSI2 on different lysates with Rabbit anti-MSI2 antibody (<a href="/products/ER1706-59" style="font-weight: bold;text-decoration: underline;">ER1706-59</a>) at 1/1,000 dilution.<br /><br />Lane 1: Hela-si NT cell lysate<br />Lane 2: Hela-si MSI2#1 cell lysate<br />Lane 3: Hela-si MSI2#2 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 35 kDa<br />Observed band size: 35 kDa<br /><br />Exposure time: 50 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br /><a href="/products/ER1706-59" style="font-weight: bold;text-decoration: underline;">ER1706-59</a> was shown to specifically react with MSI2 in Hela-si NT cells. No bands were observed when Hela-si MSI2 samples were tested. Hela-si NT and Hela-si MSI2 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ER1706-59" style="font-weight: bold;text-decoration: underline;">ER1706-59</a>, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, <a href="/products/ET1601-4" style="font-weight: bold;text-decoration: underline;">ET1601-4</a>, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of MSI2 on different lysates with Rabbit anti-MSI2 antibody (ER1706-59) at 1/1,000 dilution.

    Lane 1: Hela-si NT cell lysate
    Lane 2: Hela-si MSI2#1 cell lysate
    Lane 3: Hela-si MSI2#2 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 35 kDa
    Observed band size: 35 kDa

    Exposure time: 50 seconds;

    4-20% SDS-PAGE gel.

    ER1706-59 was shown to specifically react with MSI2 in Hela-si NT cells. No bands were observed when Hela-si MSI2 samples were tested. Hela-si NT and Hela-si MSI2 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ER1706-59, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • ICC staining MSI2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining MSI2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining MSI2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining MSI2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining MSI2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining MSI2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded rat epididymis tissue with Rabbit anti-MSI2 antibody (<a href="/products/ER1706-59" style="font-weight: bold;text-decoration: underline;">ER1706-59</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER1706-59" style="font-weight: bold;text-decoration: underline;">ER1706-59</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat epididymis tissue with Rabbit anti-MSI2 antibody (ER1706-59) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-59) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-MSI2 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-MSI2 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-MSI2 antibody (<a href="/products/ER1706-59" style="font-weight: bold;text-decoration: underline;">ER1706-59</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER1706-59" style="font-weight: bold;text-decoration: underline;">ER1706-59</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-MSI2 antibody (ER1706-59) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-59) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-MSI2 antibody (<a href="/products/ER1706-59" style="font-weight: bold;text-decoration: underline;">ER1706-59</a>) at 1/4,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER1706-59" style="font-weight: bold;text-decoration: underline;">ER1706-59</a>) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-MSI2 antibody (ER1706-59) at 1/4,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-59) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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