LOX-1 Rabbit Polyclonal Antibody (ER1706-53)
Catalog# ER1706-53
Rabbit Polyclonal to LOX-1
-
WB
-
IHC-P
-
IF-Cell
-
FC
-
Human
-
Mouse
Lysates/proteins at 10 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 50 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-53) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
LOX-1 Rabbit Polyclonal Antibody (ER1706-53)
Immunogen
Recombinant protein within human lox-1 aa 17-273 / 273.
Host
Rabbit
Positive Control
MCF-7 cell lysates, human lung tissue lysates, human kidney tissue, human placenta tissue, mouse brain tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
-
WB
-
1:500-1:1,000
-
IHC-P
-
1:200-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 31 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
LOX-1
SYNONYMS
C-type lectin domain family 8 member A antibody;CLEC8A antibody;hLOX 1 antibody;hLOX-1 antibody;Lectin like oxidized LDL receptor 1 antibody;Lectin like oxLDL receptor 1 antibody;Lectin type oxidized LDL receptor 1 antibody;Lectin-like oxidized LDL receptor 1 antibody;Lectin-like oxLDL receptor 1 antibody;Lectin-type oxidized LDL receptor 1 antibody;low density lipoprotein oxidized, receptor 1 antibody;LOX-1 antibody;LOXIN antibody;Olr1 antibody;OLR1_HUMAN antibody;Ox LDL receptor 1 antibody;Ox-LDL receptor 1 antibody;Oxidised low density lipoprotein (lectin like) receptor 1 antibody;Oxidized low density lipoprotein receptor 1 antibody;Oxidized low density lipoprotein receptor 1 soluble form antibody;Oxidized low-density lipoprotein receptor 1 antibody;SCARE1 antibody;Scavenger receptor class E, member 1 antibody;SLOX1 antibody;soluble form antibody;SR-EI antibody
TISSUE SPECIFICITY
Expressed at high level in endothelial cells and vascular-rich organs such as placenta, lung, liver and brain, aortic intima, bone marrow, spinal cord and substantia nigra. Also expressed at the surface of dendritic cells. Widely expressed at intermediate and low level.
POST-TRANSLATIONAL MODIFICATION
The intrachain disulfide-bonds prevent N-glycosylation at some sites.; N-glycosylated.
SUBCELLULAR LOCATION
Cell membrane. Secreted.
FUNCTION
The oxidized low density lipoprotein (lectin-like) receptor-1, Ox-LDL R-1, is a type II membrane protein that is a member of the C-type lectin family and acts as a cell-surface receptor for oxidized low density lipoprotein (Ox-LDL). Ox-LDL plays a role in early atherosclerosis, which includes the transformation of monocyte-derived macrophages to foam cells in atherosclerotic lesions. The binding of Ox-LDL to Ox-LDL R-1 may also trigger the activation of the NFkB signal transduction pathway. Ox-LDL R-1, also designated scavenger receptor class E, member 1 (SCARE1); lectin-type oxidized LDL receptor 1 (LOX-1); and CLEC8A), is expressed by vascular endothelial cells, smooth muscle cells and macrophages. It is expressed endogenously as a precursor form with N-linked high mannose carbohydrate chains and as a mature form due to further glycosylation. The N-linked glycosylation of Ox-LDL R-1 appears to be necessary for adequate transportation to the cell surface and efficient ligand binding.
CITATIONS
-
Daisong Li,et al
Integrated metabolomics and network pharmacology to reveal the mechanisms of Shexiang Baoxin pill against atherosclerosis
-
WB
-
Mouse
-
Citation
-
