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TGM6 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1706-28

TGM6 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

TGM6 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide corresponding to N-terminal of Mouse TGM6

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P, FC

Target Molecular Weight

79/71 kDa

Positive Control

293T, A549, SH-SY5Y, rat brain tissue, human kidney tissue, mouse testis tissue, mouse brain tissue, mouse spleen tissue.

Conjugation

unconjugated

RRID

AB_3069044

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

Target

Function

Transglutaminase 6 is a protein that in humans is encoded by the TGM6 gene. The protein encoded by this gene belongs to the transglutaminase superfamily. It catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. Mutations in this gene are associated with spinocerebellar ataxia type 35 (SCA35). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Background References

暂无

Sequence Similarity

Belongs to the transglutaminase superfamily. Transglutaminase family.

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: O95932 Human

SwissProt: Q6Y684 Mouse

SwissProt: F1M4T7 Rat

Synonyms

Protein-glutamine gamma-glutamyltransferase 6 antibody

TG6 antibody

TGase Y antibody

TGase-3-like antibody

TGase-6 antibody

TGM3L_HUMAN antibody

TGM6 antibody

TGY antibody

Transglutaminase Y antibody

Transglutaminase-3-like antibody

Expand

Images

  • ICC staining TGM6 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining TGM6 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining TGM6 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining TGM6 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining TGM6 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining TGM6 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TGM6 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TGM6 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-TGM6 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-TGM6 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TGM6 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TGM6 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-TGM6 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-TGM6 antibody. Counter stained with hematoxylin.

  • Western blot analysis of TGM6 on different lysates with Rabbit anti-TGM6 antibody (<a href="/products/ER1706-28" style="font-weight: bold;text-decoration: underline;">ER1706-28</a>) at 1/1,000 dilution.<br /><br />Lane 1: MOLT-4 cell lysate<br />Lane 2: NIH/3T3 cell lysate <br />Lane 3: Mouse kidney tissue lysate<br />Lane 4: Rat kidney tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Exposure time: 180 seconds; ECL: K1802<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ER1706-28" style="font-weight: bold;text-decoration: underline;">ER1706-28</a>, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 79 kDa<br />Observed band size: 71 kDa

    Western blot analysis of TGM6 on different lysates with Rabbit anti-TGM6 antibody (ER1706-28) at 1/1,000 dilution.

    Lane 1: MOLT-4 cell lysate
    Lane 2: NIH/3T3 cell lysate
    Lane 3: Mouse kidney tissue lysate
    Lane 4: Rat kidney tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Exposure time: 180 seconds; ECL: K1802

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ER1706-28, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 79 kDa
    Observed band size: 71 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"