alpha Tubulin Rabbit Polyclonal Antibody (ER130905)
Catalog# ER130905
Rabbit Polyclonal to alpha Tubulin
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WB
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IHC-P
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FC
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IF-Cell
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IF-Tissue
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Human
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Mouse
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Rat
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Zebrafish
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: PC-12 cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: Daudi cell lysate (20 µg/Lane)
Lane 5: Jurkat cell lysate (20 µg/Lane)
Lane 6: A431 cell lysate (20 µg/Lane)
Lane 7: K-562 cell lysate (20 µg/Lane)
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER130905) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
alpha Tubulin Rabbit Polyclonal Antibody (ER130905)
Immunogen
Synthetic peptide within human alpha-tubulin aa 402-451 / 451.
Host
Rabbit
Positive Control
HeLa cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, Daudi cell lysate, Jurkat cell lysate, A431 cell lysate, K-562 cell lysate, HepG2, Hela, Neuro-2a, C6, human stomach tissue, human tonsil tissue, mouse pancreas tissue, rat large intestine tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:5,000-1:50,000
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IHC-P
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1:200-1:500
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IF-Cell
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1:200-1:500
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IF-Tissue
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1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 50 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
alpha Tubulin
SYNONYMS
TUBA1 antibody;TUBA1A antibody;TUBA2 antibody;TUBA3 antibody;TUBA3C antibody;TUBA4A antibody;Tubulin, alpha 1a antibody;Tubulin, alpha 3c antibody;Tubulin, alpha 4a antibody
SEQUENCE SIMILARITIES
Belongs to the tubulin family.
POST-TRANSLATIONAL MODIFICATION
Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold.; Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).; Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly.; Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability.
SUBCELLULAR LOCATION
Cytoplasm, Cytoskeleton, Microtubule.
FUNCTION
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
CITATIONS
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Ren, Q., Wang, B., Guo, F., Huang, R., Tan, Z., Ma, L., & Fu, P.
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Mouse
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Tang, C., Jin, M., Ma, B., Cao, B., Lin, C., Xu, S., Li, J., & Xu, Q.
RGS2 promotes estradiol biosynthesis by trophoblasts during human pregnancy
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Human
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Mouse
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Bao Jiayang,et al
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Human
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Identification of ANXA1 as a Novel Upstream Negative Regulator of Notch1 Function in AML
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IF
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IHC
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Eriocheir sinensis
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Xu Y, Qu M, He Y, et al
Smurf1 polyubiquitinates on K285/K282 of the kinases Mst1/2 to attenuate their tumor-suppressor functions
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WB
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Human
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Mouse
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Citation
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Wang, X., Shao, G., Hong, X., Shi, Y., Zheng, Y., Yu, Y., & Fu, C.
Targeting Annexin A1 as a Druggable Player to Enhance the Anti-Tumor Role of Honokiol in Colon Cancer through Autophagic Pathway
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WB
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Human
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Citation
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Dong, J., Zhong, T., Xu, Z., Chen, H., Wang, X., Yang, L., Lou, Z., Xu, Y., Hou, T., Xu, R., Zhu, W., & Shao, J.
Identification of Monobenzone as a Novel Potential Anti-Acute Myeloid Leukaemia Agent That Inhibits RNR and Suppresses Tumour Growth in Mouse Xenograft Model
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Shu, Y., Zou, C., Cai, Y., He, Q., Wu, X., Zhu, H., Qv, M., Chao, Y., Xu, C., Tang, L., & Wu, X.
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WB
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Mouse
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Citation
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Guo Fangzhou,et al
Identification and validation of prognostic genes and immune landscape signatures based on a fatty acid oxidation‑related risk score model in glioma
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WB
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Human
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PGK1-coupled HSP90 stabilizes GSK3β expression to regulate the stemness of breast cancer stem cells
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WB
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Human
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Citation
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Liu, Q., Guo, L., Qi, H., Lou, M., Wang, R., Hai, B., Xu, K., Zhu, L., Ding, Y., Li, C., Xie, L., Shen, J., Xiang, X., & Shao, J.
A MYBL2 complex for RRM2 transactivation and the synthetic effect of MYBL2 knockdown with WEE1 inhibition against colorectal cancer. Cell death & disease, 12(7), 683.
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Mouse
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Citation
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