ApoER2 Mouse Monoclonal Antibody [13B2]
Catalog# EM1902-30
ApoER2 Mouse Monoclonal Antibody [13B2]
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WB
-
IHC-P
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Human
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unconjugated
Overview
Product Name
ApoER2 Mouse Monoclonal Antibody [13B2]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human ApoER2 aa 47-275 / 963.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 106 kDa
Positive Control
A549 cell lysate, SHSY5Y cell lysate, SKBR-3 cell lysate, U937 cell lysate, Hela, A549, human testis tissue.
Conjugation
unconjugated
Clone Number
13B2
RRID
Product Features
Form
Liquid
Concentration
2 mg/ml.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG2a
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500
-
IHC-P
-
1:400
Target
Function
Cell surface receptor for Reelin (RELN) and apolipoprotein E (apoE)-containing ligands. LRP8 participates in transmitting the extracellular Reelin signal to intracellular signaling processes, by binding to DAB1 on its cytoplasmic tail. Reelin acts via both the VLDL receptor (VLDLR) and LRP8 to regulate DAB1 tyrosine phosphorylation and microtubule function in neurons. LRP8 has higher affinity for Reelin than VLDLR. LRP8 is thus a key component of the Reelin pathway which governs neuronal layering of the forebrain during embryonic brain development. Binds the endoplasmic reticulum resident receptor-associated protein (RAP). Binds dimers of beta 2-glycoprotein I and may be involved in the suppression of platelet aggregation in the vasculature. Highly expressed in the initial segment of the epididymis, where it affects the functional expression of clusterin and phospholipid hydroperoxide glutathione peroxidase (PHGPx), two proteins required for sperm maturation. May also function as an endocytic receptor. Not required for endocytic uptake of SEPP1 in the kidney which is mediated by LRP2 (By similarity). Together with its ligand, apolipoprotein E (apoE), may indirectly play a role in the suppression of the innate immune response by controlling the survival of myeloid-derived suppressor cells (By similarity).
Background References
1. Andersen O.M.et.al.Differential binding of ligands to the apolipoprotein E receptor 2.Biochemistry 42:9355-9364(2003).
Sequence Similarity
Belongs to the LDLR family.
Tissue Specificity
Expressed mainly in brain and placenta. Also expressed in platelets and megakaryocytic cells. Not expressed in the liver.
Post-translational Modification
O-glycosylated. Some alternatively spliced isoforms lack the O-linked sugar domain (By similarity).; Undergoes sequential, furin and gamma-secretase dependent, proteolytic processing, resulting in the extracellular release of the entire ligand-binding domain as a soluble polypeptide and in the intracellular domain (ICD) release into the cytoplasm. The gamma-secretase-dependent proteolytical processing occurs after the bulk of the extracellular domain has been shed, in a furin-dependent manner, in alternatively spliced isoforms carrying the furin cleavage site. Hypoglycosylation (mainly hypo-O-glycosylation) leads to increased extracellular cleavage, which in turn results in accelerating release of the intracellular domain (ICD) by the gamma-secretase. The resulting receptor fragment is able to inhibit Reelin signaling and in particular the Reelin-induced DAB1 phosphorylation (By similarity).; Tyrosine phosphorylated upon apoE binding.; Ubiquitinated by MYLIP leading to degradation.
Subcellular Location
Cell membrane, Secreted.
UNIPROT
Synonyms
APOER2 antibody
Apolipoprotein E receptor 2 antibody
low density lipoprotein receptor-related protein 8 antibody
Low-density lipoprotein receptor-related protein 8 antibody
LRP-8 antibody
LRP8 antibody
LRP8_HUMAN antibody
Images
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Western blot analysis of ApoER2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-30, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A549 cell lysate
Lane 2: SHSY5Y cell lysate
Lane 3: SKBR-3 cell lysate
Lane 4: U937 cell lysate -
Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-ApoER2 antibody (EM1902-30) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-30) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
ApoER2 Recombinant Rabbit Monoclonal Antibody [JE31-73]
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Products with the same target and pathway
ApoER2 Rabbit Polyclonal Antibody
Application: WB,IHC-P,IF-Tissue
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated