LYRIC Mouse Monoclonal Antibody [6-F4]
Catalog# EM1901-61
LYRIC Mouse Monoclonal Antibody [6-F4]
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WB
-
FC
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Human
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unconjugated
Overview
Product Name
LYRIC Mouse Monoclonal Antibody [6-F4]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human LYRIC aa 6-233 / 582.
Species Reactivity
Human
Validated Applications
WB, FC
Target Molecular Weight
Predicted band size: 64 kDa
Positive Control
Jurkat cell lysate, Hela cell lysate, K562.
Conjugation
unconjugated
Clone Number
6-F4
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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FC
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1:50-1:100
Target
Function
Metadherin, also known as protein LYRIC or astrocyte elevated gene-1 protein (AEG-1) is a protein that in humans is encoded by the MTDH gene. MTDH (AEG-1) is involved in HIF-1alpha mediated angiogenesis. MTDH also interacts with SND1 and involved in RNA-induced silencing complex (RISC) and plays very important role in RISC and miRNA functions. MTDH has been shown to interact with spliceosome proteins in the cell nucleus and regulate the process of alternative splicing. MTDH induces an oncogene called Late SV40 factor (LSF/TFCP2) which is involved in thymidylate synthase (TS) induction and DNA biosynthesis synthesis. Late SV40 factor (LSF/TFCP2) enhances angiogenesis by transcriptionally up-regulating matrix metalloproteinase-9 (MMP9). MTDH acts as an oncogene in melanoma, malignant glioma, breast cancer and hepatocellular carcinoma. It is highly expressed in these cancers and helps in progression and development of these cancers. It is induced by c-Myc oncogene and plays very important role in anchorage independent growth of cancer cells. Elevated expression of the metastasis gene metadherin (MTDH), which is overexpressed in more than 40% of breast cancers, is associated with poor clinical outcomes.
Background References
1. Tong L.et. al. MTDH promotes glioma invasion through regulating miR-130b-ceRNAs. Oncotarget. 2017 Mar 14;8(11):17738-17749.
2. He R.et. al. The essential role of MTDH in the progression of HCC: a study with immunohistochemistry, TCGA, meta-analysis and in vitro investigation. Am J Transl Res. 2017 Apr 15;9(4):1561-1579.
Tissue Specificity
Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme.
Subcellular Location
Nucleus membrane, nucleolus, endoplasmic reticulum membrane, tight junction, perinuclear region, cytoplasm.
UNIPROT
Synonyms
3D3 antibody
3D3/LYRIC antibody
AEG 1 antibody
AEG-1 antibody
AEG1 antibody
Astrocyte elevated gene 1 antibody
Astrocyte elevated gene-1 protein antibody
LYRIC antibody
LYRIC/3D3 antibody
LYRIC_HUMAN antibody
ExpandImages
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Western blot analysis of LYRIC on different lysates with Rabbit anti-LYRIC antibody (EM1901-61) at 1/5,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 2: Jurkat (Human T-lymphoblastic cells) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 180 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: EM1901-61, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 64 kDa
Observed band size: 80 kDa -
Flow cytometric analysis of LYRIC was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-61, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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