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LYRIC Mouse Monoclonal Antibody [6-F4]

Usd: 350

Specification

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Catalog# EM1901-61

LYRIC Mouse Monoclonal Antibody [6-F4]

Application

  • WB

  • FC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

LYRIC Mouse Monoclonal Antibody [6-F4]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within Human LYRIC aa 6-233 / 582.

Species Reactivity

Human

Validated Applications

WB, FC

Molecular Weight

Predicted band size: 64 kDa

Positive Control

MCF-7 cell lysate, Jurkat cell lysate, Hela cell lysate, K562.

Conjugation

unconjugated

Clone Number

6-F4

RRID

AB_3068906

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein G affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • FC

  • 1:50-1:100

Target

Function

Metadherin, also known as protein LYRIC or astrocyte elevated gene-1 protein (AEG-1) is a protein that in humans is encoded by the MTDH gene. MTDH (AEG-1) is involved in HIF-1alpha mediated angiogenesis. MTDH also interacts with SND1 and involved in RNA-induced silencing complex (RISC) and plays very important role in RISC and miRNA functions. MTDH has been shown to interact with spliceosome proteins in the cell nucleus and regulate the process of alternative splicing. MTDH induces an oncogene called Late SV40 factor (LSF/TFCP2) which is involved in thymidylate synthase (TS) induction and DNA biosynthesis synthesis. Late SV40 factor (LSF/TFCP2) enhances angiogenesis by transcriptionally up-regulating matrix metalloproteinase-9 (MMP9). MTDH acts as an oncogene in melanoma, malignant glioma, breast cancer and hepatocellular carcinoma. It is highly expressed in these cancers and helps in progression and development of these cancers. It is induced by c-Myc oncogene and plays very important role in anchorage independent growth of cancer cells. Elevated expression of the metastasis gene metadherin (MTDH), which is overexpressed in more than 40% of breast cancers, is associated with poor clinical outcomes.

Background References

1. Tong L.et. al. MTDH promotes glioma invasion through regulating miR-130b-ceRNAs. Oncotarget. 2017 Mar 14;8(11):17738-17749.

2. He R.et. al. The essential role of MTDH in the progression of HCC: a study with immunohistochemistry, TCGA, meta-analysis and in vitro investigation. Am J Transl Res. 2017 Apr 15;9(4):1561-1579.

Tissue Specificity

Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme.

Subcellular Location

Nucleus membrane, nucleolus, endoplasmic reticulum membrane, tight junction, perinuclear region, cytoplasm.

UNIPROT

SwissProt: Q86UE4 Human

Synonyms

3D3 antibody

3D3/LYRIC antibody

AEG 1 antibody

AEG-1 antibody

AEG1 antibody

Astrocyte elevated gene 1 antibody

Astrocyte elevated gene-1 protein antibody

LYRIC antibody

LYRIC/3D3 antibody

LYRIC_HUMAN antibody

Expand

Images

  • Western blot analysis of LYRIC on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/EM1901-61" style="font-weight: bold;text-decoration: underline;">EM1901-61</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:5,000 dilution was used for 1 hour at room temperature.<br /><span style="font-weight: bold;">Positive control:</span> <br />Lane 1: MCF-7 cell lysate<br />Lane 2: Jurkat cell lysate<br />Lane 3: Hela cell lysate

    Western blot analysis of LYRIC on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-61, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: MCF-7 cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: Hela cell lysate

  • Flow cytometric analysis of LYRIC was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/EM1901-61" style="font-weight: bold;text-decoration: underline;">EM1901-61</a>, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of LYRIC was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-61, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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