alpha Actinin Mouse Monoclonal Antibody [A1G2] (EM1901-52)
Catalog# EM1901-52
Mouse Monoclonal to alpha Actinin
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Rat
Lane 1: HeLa cell lysate
Lane 2: A431 cell lysate
Lane 3: A549 cell lysate
Lane 4: MCF7 cell lysate
Lane 5: PC-12 cell lysate
Lane 6: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 103 kDa
Observed band size: 103 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-52) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
alpha Actinin Mouse Monoclonal Antibody [A1G2] (EM1901-52)
Immunogen
Recombinant protein within human actn1 aa 388-619 / 892.
Host
Mouse
Positive Control
HeLa cell lysate, A431 cell lysate, A549 cell lysate, MCF7 cell lysate, PC-12 cell lysate, C6 cell lysate, A431, SiHa, JAR, human lung tissue, human liver carcinoma tissue, human skin tissue, human breast tissue, human breast carcinoma tissue, human kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A1G2
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:50-1:100
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IHC-P
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1:50-1:500
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FC
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1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 103 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
alpha Actinin
SYNONYMS
actinin 1 smooth muscle antibody;Actinin alpha 1 antibody;actinin, alpha 1 antibody;ACTN 1 antibody;Actn1 antibody;ACTN1_HUMAN antibody;Alpha Actinin 1 antibody;Alpha actinin cytoskeletal isoform antibody;Alpha-actinin cytoskeletal isoform antibody;Alpha-actinin-1 antibody;BDPLT15 antibody;F actin cross linking protein antibody;F-actin cross-linking protein antibody;FLJ40884 antibody;FLJ54432 antibody;Non muscle alpha actinin 1 antibody;Non-muscle alpha-actinin-1 antibody
SEQUENCE SIMILARITIES
Belongs to the alpha-actinin family.
SUBCELLULAR LOCATION
Cell membrane, cytoskeleton, Z line, cell junction, ruffle.
FUNCTION
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.
CITATIONS
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Biao, T., Cai-Feng, H., Xiao-Hong, L., Xiao-Li, C., Wen-Bei, L., Jun, W., Chao, C., & Tao, Y.
From Bowen disease to cutaneous squamous cell carcinoma: eight markers were verified from transcriptomic and proteomic analyses
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WB
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Human
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Citation
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Yu, C., Yue, Z., Shi, M., Jiang, L., Chen, S., Yao, M., Yu, Q., Wu, X., Zhang, H., Yao, F., Wang, C., Sun, H., & Li, J.
An Intrapericardial Injectable Hydrogel Patch for Mechanical-Electrical Coupling with Infarcted Myocardium
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IF
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Rat
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Citation
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