USP21 Mouse Monoclonal Antibody [16E1] (EM1901-37)
Catalog# EM1901-37
Mouse Monoclonal to USP21
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Rat
Positive control:
Lane 1: SH-SY5Y cell lysates
Lane 2: K562 cell lysates
Specifications
Product Type
Mouse monoclonal primary
Product Name
USP21 Mouse Monoclonal Antibody [16E1] (EM1901-37)
Immunogen
Recombinant protein within human usp21 aa 303-453 / 565.
Host
Mouse
Positive Control
SH-SY5Y cell lysates, K562 cell lysates, SH-SY5Y , rat seminal vesicle tissue, human liver tissue, human kidney tissue, human small intestine tissue, mouse heart tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
16E1
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IF-Cell
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1:100
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 63 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
USP21
SYNONYMS
Deubiquitinating enzyme 21 antibody;deubiquitinating enzyme 23 antibody;EC 3.1.2.15 antibody;ESTM28 antibody;MGC3394 antibody;NEDD8-specific protease antibody;Ubiquitin carboxyl terminal hydrolase 21 antibody;ubiquitin carboxyl terminal hydrolase 23 antibody;Ubiquitin carboxyl-terminal hydrolase 21 antibody;ubiquitin specific peptidase 21 antibody;Ubiquitin specific processing protease 21 antibody;ubiquitin specific protease 16 antibody;ubiquitin specific protease 21 antibody;ubiquitin specific protease 23 antibody;Ubiquitin thioesterase 21 antibody;Ubiquitin thiolesterase 21 antibody;ubiquitin thiolesterase 23 antibody;ubiquitin-specific protease 23, formerly antibody;Ubiquitin-specific-processing protease 21 antibody;UBP21_HUMAN antibody;USP16 antibody;Usp21 antibody;USP23 antibody;USP23, formerly antibody;W53272 antibody
SEQUENCE SIMILARITIES
Belongs to the peptidase C19 family. USP21 subfamily.
TISSUE SPECIFICITY
Highly expressed in heart, pancreas and skeletal muscle. Also expressed in brain, placenta, liver and kidney, and at very low level in lung.
SUBCELLULAR LOCATION
Nucleus, cytoplasm.
FUNCTION
USP21 (Ubiquitin Specific Peptidase 21) is a Protein Coding gene. Diseases associated with USP21 include Neurodevelopmental Disorder With Spasticity And Poor Growth. Among its related pathways are TNF signaling (REACTOME) and Integrated Breast Cancer Pathway. Gene Ontology (GO) annotations related to this gene include transcription coactivator activity and cysteine-type peptidase activity. An important paralog of this gene is USP2. Deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A releaves the repression of di- and trimethylation of histone H3 at 'Lys-4', resulting in regulation of transcriptional initiation. Regulates gene expression via histone H2A deubiquitination. Also capable of removing NEDD8 from NEDD8 conjugates but has no effect on Sentrin-1 conjugates. Deubiquitinates BAZ2A/TIP5 leading to its stabilization.
CITATIONS
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Yang, S., Yan, H., Wu, Y., Shan, B., Zhou, D., Liu, X., Mao, X., Zhou, S., Zhao, Q., & Xia, H.
Deubiquitination and Stabilization of PD-L1 by USP21
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IHC
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Human
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Citation
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