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NQO1 Mouse Monoclonal Antibody [13A1]

Usd: 350

Specification

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Catalog# EM1801-21

NQO1 Mouse Monoclonal Antibody [13A1]

Application

  • WB

Reactivity

  • Human

Predicted reactivity

  • Rat

Predicted species support after-sales service
Conjugation

  • unconjugated

Overview

Product Name

NQO1 Mouse Monoclonal Antibody [13A1]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within Human NQO1 aa 1-274 / 274.

Species Reactivity

Human (Predicted: Rat)

Predicted species support after-sales service

Validated Applications

WB

Molecular Weight

Predicted band size: 31 kDa

Positive Control

A549 cell lysate, SiHa cell lysate, HepG2 cell lysate, MCF7 cell lysate, HeLa cell lysate.

Conjugation

unconjugated

Clone Number

13A1

RRID

AB_3068844

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG2b

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:2,000

Target

Function

NQO1, a 274 amino acid protein, is ubiquitously expressed, but the expression level varies among tissues. NQO1 gene expression is coordinately induced in response to xenobiotics, antioxidants, heavy metals and radiation. The antioxidant response element (ARE) in the NQO1 gene promoter is essential for expression and coordinated induction of NQO1. ARE activation by tert-butylhydroquinone is dependent on PI3-kinase, which lies upstream of Nrf2. Nrf2, c-Jun, Nrf1, Jun-B and Jun-D bind to the ARE and regulate expression and induction of NQO1 gene. Maf-Maf homodimers and possibly Maf-Nrf2 heterodimers play a role in negative regulation of ARE-mediated transcription, but Maf-Nrf1 heterodimers fail to bind with the NQO1 gene ARE and do not repress NQO1 transcription.

Background References

1. Jaiswal A K et al. Human NAD(P)H:quinone oxidoreductase (NQO1) gene structure and induction by dioxin. Biochemistry 30:10647-10653 (1991).

2. Guryanova O A et al. Actin cytoskeleton remodeling by the alternatively spliced isoform of PDLIM4/RIL protein. J Biol Chem 286:26849-26859 (2011).

Sequence Similarity

Belongs to the NAD(P)H dehydrogenase (quinone) family.

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P15559 Human

SwissProt: P05982 Rat

Synonyms

Azoreductase antibody

Cytochrome b 5 reductase antibody

DHQU antibody

DIA 4 antibody

DIA4 antibody

Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) antibody

Diaphorase (NADH/NADPH) antibody

Diaphorase 4 antibody

Dioxin inducible 1 antibody

DT diaphorase antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of NQO1 on different lysates with Mouse anti-NQO1 antibody (<a href="/products/EM1801-21" style="font-weight: bold;text-decoration: underline;">EM1801-21</a>) at 1/2,000 dilution.<br /><br />Lane 1: A549 cell lysate<br />Lane 2: SiHa cell lysate<br />Lane 3: HepG2 cell lysate<br />Lane 4: MCF7 cell lysate<br />Lane 5: HeLa cell lysate<br />Lane 6: Saos-2 cell lysate (low expression)<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 31 kDa<br />Observed band size: 31 kDa<br /><br />Exposure time: 5 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM1801-21" style="font-weight: bold;text-decoration: underline;">EM1801-21</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of NQO1 on different lysates with Mouse anti-NQO1 antibody (EM1801-21) at 1/2,000 dilution.

    Lane 1: A549 cell lysate
    Lane 2: SiHa cell lysate
    Lane 3: HepG2 cell lysate
    Lane 4: MCF7 cell lysate
    Lane 5: HeLa cell lysate
    Lane 6: Saos-2 cell lysate (low expression)

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 31 kDa
    Observed band size: 31 kDa

    Exposure time: 5 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-21) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of NQO1 on different lysates with Mouse anti-NQO1 antibody (<a href="/products/EM1801-21" style="font-weight: bold;text-decoration: underline;">EM1801-21</a>) at 1/2,000 dilution.<br /><br />Lane 1: HCT 116-si NT cell lysate<br />Lane 2: HCT 116-si NQO1 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 31 kDa<br />Observed band size: 31 kDa<br /><br />Exposure time: 3 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM1801-21" style="font-weight: bold;text-decoration: underline;">EM1801-21</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of NQO1 on different lysates with Mouse anti-NQO1 antibody (EM1801-21) at 1/2,000 dilution.

    Lane 1: HCT 116-si NT cell lysate
    Lane 2: HCT 116-si NQO1 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 31 kDa
    Observed band size: 31 kDa

    Exposure time: 3 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-21) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"