TGG2 Mouse Monoclonal Antibody [6H1]
Catalog# EM1701-93
TGG2 Mouse Monoclonal Antibody [6H1]
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WB
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IHC-P
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IF-Tissue
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Arabidopsis thaliana
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unconjugated
Overview
Product Name
TGG2 Mouse Monoclonal Antibody [6H1]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within thaliana TGG2 aa 350-400.
Species Reactivity
Arabidopsis thaliana
Validated Applications
WB, IHC-P, IF-Tissue
Target Molecular Weight
Predicted band size: 63 kDa
Positive Control
Arabidopsis thaliana lysates, Arabidopsis thaliana.
Conjugation
unconjugated
Clone Number
6H1
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IHC-P
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1:200
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IF-Tissue
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1:50-1:200
Target
Function
May degrade glucosinolates (glucose residue linked by a thioglucoside bound to an amino acid derivative) to glucose, sulfate and any of the products: thiocyanates, isothiocyanates, nitriles, epithionitriles or oxazolidine-2-thiones. These toxic degradation products can deter insect herbivores. Seems to function in abscisic acid (ABA) and methyl jasmonate (MeJA) signaling in guard cells. Functionally redundant with TGG1.
Background References
1. Zhou C et al. Properties of β-thioglucoside hydrolases (TGG1 and TGG2) from leaves of Arabidopsis thaliana. Plant Sci. 191-192:82-92 (2012).
2. Islam MM et al. Myrosinases, TGG1 and TGG2, redundantly function in ABA and MeJA signaling in Arabidopsis guard cells. Plant Cell Physiol 50:1171-1175 (2009).
Sequence Similarity
Belongs to the glycosyl hydrolase 1 family.
Tissue Specificity
Expressed in phloem-associated cells.
Subcellular Location
Chloroplast. Cytosol.Secreted.
Images
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Western blot analysis of TGG2 on Arabidopsis thaliana lysate with Mouse anti-TGG2 antibody (EM1701-93) at 1/1,000 dilution.
Lysates/proteins at 10 µg/Lane.
Exposure time: 30 seconds
Predicted band size: 63 kDa
Observed band size: 63 kDa -
IF staining TGG2 (green) in Arabidopsis thaliana (longitudinal section). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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IF staining TGG2 (green) in Arabidopsis thaliana (cross-section). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded Arabidopsis thaliana tissue using anti-TGG2 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"