PLGF Mouse Monoclonal Antibody [3G2]
Catalog# EM1701-90
PLGF Mouse Monoclonal Antibody [3G2]
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WB
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IHC-P
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IF-Cell
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
PLGF Mouse Monoclonal Antibody [3G2]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human PLGF aa 1-221.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IF-Cell
Target Molecular Weight
Predicted band size: 25 kDa
Positive Control
JAR (Human placental choriocarcinoma cell) cell lysate, HepG2, LOVO, rat kidney tissue, mouse testis tissue.
Conjugation
unconjugated
Clone Number
3G2
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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WB
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1:500
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IF-Cell
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1:50-1:100
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IHC-P
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1:50-1:200
Target
Function
Placental growth factor (PlGF) is a protein that in humans is encoded by the PGF gene. Placental growth factor is a member of the VEGF (vascular endothelial growth factor) sub-family - a key molecule in angiogenesis and vasculogenesis, in particular during embryogenesis. The main source of PlGF during pregnancy is the placental trophoblast. PGF is also expressed in many other tissues, including the villous trophoblast. The placental growth factor gene (PGF) is a protein-coding gene and a member of the vascular endothelial growth factor (VEGF) family. PGF is expressed only in human umbilical vein endothelial cells, and the placenta. PGF is ultimately associated with angiogenesis. Specifically, PGF plays a role in trophoblast growth and differentiation. Trophoblast cells, specifically extravillous trophoblast cells, are responsible for invading the uterine wall and the maternal spiral arteries. The extravillous trophoblast cells produce a blood vessel of larger diameter for the developing fetus that is independent of maternal vasoconstriction. This is essential for increased blood flow and reduced resistance. Proper development of blood vessels in the placenta is crucial for the higher blood requirement of the fetus later in pregnancy. Under normal physiologic conditions, PGF is also expressed at a low level in other organs including the heart, lung, thyroid, and skeletal muscle.
Background References
1. Rolny C et al. HRG inhibits tumor growth and metastasis by inducing macrophage polarization and vessel normalization through downregulation of PlGF. Cancer Cell 19:31-44 (2011).
2. Park J E et al. Placenta growth factor. Potentiation of vascular endothelial growth factor bioactivity, in vitro and in vivo, and high affinity binding to Flt-1 but not to Flk-1/KDR. J Biol Chem 269:25646-25654 (1994).
Sequence Similarity
Belongs to the PDGF/VEGF growth factor family.
Tissue Specificity
While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas.
Post-translational Modification
N-glycosylated.
Subcellular Location
Secreted.
Synonyms
D12S1900 antibody
Pgf antibody
PGFL antibody
PIGF antibody
Placenta growth factor antibody
Placental growth factor antibody
Placental growth factor, vascular endothelial growth factor related protein antibody
PlGF 2 antibody
PlGF antibody
PLGF_HUMAN antibody
ExpandImages
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Western blot analysis of PLGF on JAR (Human placental choriocarcinoma cell) cell lysate with Rabbit anti-PLGF antibody (EM1701-90) at 1/500 dilution.
Lysates/proteins at 10 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: EM1701-90, 1/500 in 5% NFDM/TBST, 2 hours at room temperature
Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 25 kDa
Observed band size: 25 kDa -
ICC staining PLGF (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining PLGF (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PLGF antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PLGF antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
PLGF Recombinant Rabbit Monoclonal Antibody [JA63-15]
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Products with the same target and pathway
Human PLGF Recombinant Rabbit Monoclonal Antibody [PSH23-85] - BSA and Azide free (Capture)
Application: ELISA(Cap)
Reactivity: Human
Conjugate: unconjugated
Biotin Conjugated Human PLGF Recombinant Rabbit Monoclonal Antibody [PSH23-86] - Detector
Application: ELISA(Det),ELISA
Reactivity: Human
Conjugate: Biotin
PLGF Rabbit Polyclonal Antibody
Application: IHC-P,FC
Reactivity: Human,Mouse
Conjugate: unconjugated
PLGF Recombinant Rabbit Monoclonal Antibody [JA63-15]
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Human PLGF Recombinant Rabbit Monoclonal Antibody [PSH23-86] - BSA and Azide free (Detector)
Application: ELISA(Det)
Reactivity: Human
Conjugate: unconjugated
