AMACR Mouse Monoclonal Antibody [6F5]
Catalog# EM1701-80
AMACR Mouse Monoclonal Antibody [6F5]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
AMACR Mouse Monoclonal Antibody [6F5]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human AMACR aa 1-382 / 382.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 42 kDa
Positive Control
SiHa cell lysate, human liver tissue lysate, human kidney tissue, human liver tissue, human prostate carcinoma tissue, human colon carcinoma tissue, mouse kidney tissue, rat kidney tissue, A549.
Conjugation
unconjugated
Clone Number
6F5
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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WB
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1:500-1:2,000
Target
Function
AMACR, is an enzyme that responsible for converting (2R)-methylacyl-CoA esters to their (2S)-methylacyl-CoA epimers and known substrates, including coenzyme A esters of pristanic acid (mostly derived from phytanic acid, a 3-methyl branched-chain fatty acid that is abundant in the diet) and bile acids derived from cholesterol. Both decreased and increased levels of the enzyme in humans are linked with diseases. Reduction of the protein level or activity results in the accumulation of (2R)-methyl fatty acids such as bile acids which causes neurological symptoms. Increased levels of AMACR protein concentration and activity are associated with prostate cancer, and the enzyme is used widely as a biomarker (known in cancer literature as P504S) in biopsy tissues. Increased levels of AMACR are also associated with some breast, colon, and other cancers, but it is unclear exactly what the role of AMACR is in these cancers. This AMACR antibody is intended for qualified laboratories to qualitatively identify by light microscopy the presence of associated antigens in sections of formalinfixed, paraffin-embedded tissue sections using IHC test methods.
Background References
1. Ouyang B et al. α-Methylacyl-CoA racemase spliced variants and their expression in normal and malignant prostate tissues. Urology 77(1):249.e1-7 (2011).
2. Ferdinandusse S et al. Mutations in the gene encoding peroxisomal alpha-methylacyl-CoA racemase cause adult-onset sensory motor neuropathy. Nature Genetics 24(2):188-91 (2000).
Sequence Similarity
Belongs to the CoA-transferase III family.
Subcellular Location
Mitochondrion. Peroxisome.
Synonyms
2 arylpropionyl CoA epimerase antibody
2 methylacyl CoA racemase antibody
2-methylacyl-CoA racemase antibody
Alpha methylacyl CoA racemase antibody
Alpha methylacyl Coenzyme A racemase antibody
Alpha methylacyl-CoA racemase deficiency, included antibody
Alpha-methylacyl-CoA racemase antibody
Amacr antibody
AMACR deficiency, included antibody
AMACR_HUMAN antibody CBAS4 antibody
ExpandImages
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Western blot analysis of AMACR on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1701-80, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SiHa cell lysate
Lane 2: Human liver tissue lysate
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"