Bmi1 Mouse Monoclonal Antibody [A11-C11]

Function

In Drosophila, Polycomb (Pc-g) gene family encodes chromatin proteins that are required for the repression of homeotic loci in embryonic development. Mel-18 and Bmi-1, mammalian homologs of Drosophila Pc-g group proteins, are similarly expressed during development and implicated in the regulation of gene expression, axial skeleton development, control of proliferation and survival of haematopoietic cells. Mel-18 directly binds to DNA through a RING-finger motif and preferentially associates with juxtaposed enhancer elements on various genes, including Bcl-2, c-Myc and Hox. Mel-18 is an immediate early response gene within the c-Myc/Cdc25 signaling cascade that exhibits tumor suppressor activity and negatively regulates cell cycle progression by blocking S phase entry. Alternatively, Bmi-1 has been identified as a potent oncogene as it contributes to the transcriptional activation of genes implicated in early lymphoid development. Proviral activation of Bmi-1 expression corresponds to enhanced gene-specific activation of other proto-oncogenes, including c-Myc and Pim, subsequently resulting in the progression of lymphomagenesis.

Background References

1. Wang H et al. Role of histone H2A ubiquitination in Polycomb silencing. Nature 431:873-878 (2004).

2. Cao R et al. Role of Bmi-1 and Ring1A in H2A ubiquitylation and Hox gene silencing. Mol Cell 20:845-854 (2005).

Post-translational Modification

Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation.

Subcellular Location

Cytoplasm. Nucleus.

UNIPROT

SwissProt: P35226 Human

SwissProt: P25916 Mouse

Synonyms