PLEKHH1 Mouse Monoclonal Antibody [3-E11]
Catalog# EM1701-48
PLEKHH1 Mouse Monoclonal Antibody [3-E11]
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WB
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IHC-P
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Human
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Mouse
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unconjugated
Overview
Product Name
PLEKHH1 Mouse Monoclonal Antibody [3-E11]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human PLEKHH1 aa 1,059-1,364 / 1,364.
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 151 kDa
Positive Control
MCF-7, A431, SH-SY5Y, human kidney tissue, mouse kidney tissue.
Conjugation
unconjugated
Clone Number
3-E11
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG2b
Purification Method
Protein G affinity purified.
Application Dilution
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WB
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1:500
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IHC-P
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1:50-1:200
Target
Function
PLEKHH1 (Pleckstrin Homology, MyTH4 And FERM Domain Containing H1) is a Protein Coding gene. Diseases associated with PLEKHH1 include Gestational Trophoblastic Neoplasm. Critical component of the guidance pathway underlying endothelial cell migration and blood vessel patterning. Involved in mediating membrane localization of ephrin proteins, which have been shown to provide guidance cues for endothelial cell migration.
Background References
1. Ota T et al. Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet 36:40-45 (2004).
Subcellular Location
Cytoskeleton.
Synonyms
KIAA1200 antibody
PH domain containing family H member 1 antibody
Pleckstrin homology domain containing, family H (with MyTH4 domain) member 1 antibody
pleckstrin homology domain-containing family H member 1 antibody
Images
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Western blot analysis of PLEKHH1 on A431 (Human epidermoid carcinoma skin squamous cell) lysates with Mouse anti-PLEKHH1 antibody (EM1701-48) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Exposure time: 1 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: EM1701-48, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 151.2 kDa
Observed band size: 35 kDa -
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PLEKHH1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PLEKHH1 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"