CD134 Mouse Monoclonal Antibody [2H1] (EM1701-24)
Catalog# EM1701-24
Mouse Monoclonal to CD134/OX40
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WB
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IHC-P
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IF-Cell
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FC
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Human
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Mouse
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Rat
Lysates/proteins at 20 µg/Lane.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-24) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
CD134 Mouse Monoclonal Antibody [2H1] (EM1701-24)
Immunogen
Recombinant fragment within human cd134/ ox40l receptor aa 59-205.
Host
Mouse
Positive Control
A549 cells, Hela cells, HepG2 cells, rat lung tissue, rat spleen tissue, human tonsil tissue, human spleen tissue, mouse spleen tissue, HL-60 cells, mouse lung tissue lysates.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
2H1
RRID
APPLICATION DILUTION
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WB
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1:500
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 29 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
CD134/OX40
SYNONYMS
ACT 35 antibody;ACT35 antibody;ACT35 antigen antibody;ATC35 antigen antibody;CD 134 antibody;CD134 antibody;CD134 antigen antibody;IMD16 antibody;Lymphoid activation antigene ACT35 antibody;OX 40 antibody;OX40 antibody;OX40 antigen antibody;OX40 cell surface antigen antibody;OX40 homologue antibody;OX40L receptor antibody;TAX transcriptionally activated glycoprotein 1 receptor antibody;TAX transcriptionally-activated glycoprotein 1 receptor antibody;TNF receptor superfamily member 4 antibody;TNFRSF 4 antibody;TNFRSF4 antibody;TNR4_HUMAN antibody;Tumor necrosis factor receptor superfamily member 4 antibody;Txgp 1l antibody;Txgp1 antibody;Txgp1l antibody
SUBCELLULAR LOCATION
Membrane.
FUNCTION
Ox40 (also designated CD134 and Ox40R), is a member of the tumor necrosis factor receptor (TNFR) family. Ox40 is involved in coordinating CD4 T cell selection, migration and cytokine differentiation in T helper (Th)1 and Th2 cells. Ox40 is also involved in the stimulation of T cells, T cell-dependent humoral response and generation of optimal CD4+ T cell responses in vivo and in vitro. Ox40 is expressed on activated CD4+ T lymphocytes and its ligand, Ox40L, is found preferentially on activated B cells. Engagement of Ox40 with its ligand, Ox40L, delivers a strong costimulatory signal to effector T cells. Members of the TNFR superfamily are critically involved in the regulation of infections, inflammation, autoimmune diseases and tissue homeostasis.
CITATIONS
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Safi, M., Jin, C., Aldanakh, A., Feng, P., Qin, H., Alradhi, M., Zhang, L., Zhang, J., Adlat, S., Zhao, Y., & Liu, J.
Immune checkpoint inhibitor (ICI) genes and aging in malignant melanoma patients: a clinicogenomic TCGA study
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IHC-P
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Human
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Citation
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