TIM 3 Mouse Monoclonal Antibody [1E5] (EM1701-20)
Catalog# EM1701-20
Mouse Monoclonal to Tim3
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WB
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IF-Cell
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IHC-P
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FC
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Human
Lane 1: THP-1 cell lysate (no heat)
Lane 2: A549 cell lysate (no heat)
Lane 3: HepG2 cell lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 30 µg/Lane.
Predicted band size: 33 kDa
Observed band size: 42 kDa
Exposure time: 5 minutes 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-20) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
TIM 3 Mouse Monoclonal Antibody [1E5] (EM1701-20)
Immunogen
Recombinant protein with human tim 3 1-89 / 301.
Host
Mouse
Positive Control
THP-1 cell lysate, A549 cell lysate, HepG2 cell lysate, human lung cancer tissue, human spleen tissue, human tonsil tissue, 293T, Daudi.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
1E5
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:100-1:200
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FC
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1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 33 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
Tim3
SYNONYMS
CD366 antibody;FLJ14428 antibody;HAVcr-2 antibody;Havcr2 antibody;HAVR2_HUMAN antibody;Hepatitis A virus cellular receptor 2 antibody;Kidney injury molecule 3 antibody;KIM 3 antibody;KIM3 antibody;T cell immunoglobulin and mucin domain containing 3 antibody;T cell immunoglobulin mucin 3 antibody;T-cell immunoglobulin and mucin domain-containing protein 3 antibody;T-cell immunoglobulin mucin family member 3 antibody;T-cell immunoglobulin mucin receptor 3 antibody;T-cell membrane protein 3 antibody;Tim 3 antibody;TIM-3 antibody;TIM3 antibody;TIMD-3 antibody;TIMD3 antibody
SEQUENCE SIMILARITIES
Belongs to the immunoglobulin superfamily. TIM family.
TISSUE SPECIFICITY
Expressed in T-helper type 1 (Th1) lymphocytes. Expressed on regulatory T (Treg) cells after TCR stimulation. Expressed in dendritic cells and natural killer (NK) cells. Expressed in epithelial tissues. Expression is increased on CD4+ and CD8+ T-cells in chronic hepatitis C virus (HCV) infection. In progressive HIV-1 infection, expression is up-regulated on HIV-1-specific CD8 T-cells.
POST-TRANSLATIONAL MODIFICATION
O-glycosylated with core 1 or possibly core 8 glycans.; Phosphorylated on tyrosine residues; modestly increased after TCR/CD28 stimulation. Can be phosphorylated in the cytoplasmatic domain by FYN (By similarity). Phosphorylation at Tyr-265 is increased by stimulation with ligand LGALS9.
SUBCELLULAR LOCATION
Membrane. Cell junction.
FUNCTION
Cell surface receptor implicated in modulating innate and adaptive immune responses. Generally accepted to have an inhibiting function. Reports on stimulating functions suggest that the activity may be influenced by the cellular context and/or the respective ligand. Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. In CD8+ cells attenuates TCR-induced signaling, specifically by blocking NF-kappaB and NFAT promoter activities resulting in the loss of IL-2 secretion. The function may implicate its association with LCK proposed to impair phosphorylation of TCR subunits, and/or LGALS9-dependent recruitment of PTPRC to the immunological synapse.
CITATIONS
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Dr Jinsong Wang;Dr Shenming Wang
TIM-3 inhibits PDGF-BB-induced atherogenic responses in human artery vascular smooth muscle cells
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WB
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IHC
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Human
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Citation
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