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IMP3 Mouse Monoclonal Antibody [C2-E4]

Usd: 185

Specification

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Catalog# EM1701-15

IMP3 Mouse Monoclonal Antibody [C2-E4]

Application

  • WB

  • IHC-P

  • IF-Cell

  • FC

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

IMP3 Mouse Monoclonal Antibody [C2-E4]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within Human IMP-3 aa 352-579 / 579.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Cell, FC, IP

Molecular Weight

Predicted band size: 64 kDa

Positive Control

HeLa cell lysate, 293T cell lysate, mouse placenta tissue lysate, rat placenta tissue lysate, Hela, SH-SY5Y, rat brain tissue, human liver cancer tissue, human placenta tissue, mouse testis tissue.

Conjugation

unconjugated

Clone Number

C2-E4

RRID

AB_3068743

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IF-Cell

  • 1:50

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • IP

  • 1-2μg/sample

Target

Function

Insulin like growth factor 2 mRNA binding proteins (IGF2BPs) bind RNA and influence RNA synthesis and metabolism. IGF2BP1, also known as coding region determinant-binding protein/insulin-like growth factor II mRNA-binding protein (CRD-BP), IMP1 or VICKZ1; IGF2BP2 (IMP2, VICKZ2, p62); and IGF2BP2 (IMP3, KOC1, VICKZ3) contain a unique combination of RNA recognition motifs and four hnRNP K homology domains. IGF2BP1 is abundant in embryonal tissues and is expressed in 81% of colon cancers, 73% of sarcomas and 58.5% of breast cancers. It recognizes c-Myc, IGF-II and t mRNAs, and H19 RNA, and plays a major role in proliferation of K-562 cells by an IGF-II-dependent mechanism. IGF2BP2 binds the 5' UTR of IGF-II mRNA and influences tumor cell growth, in which IGF2BP2 is associated with apoptosis induced by tretinoin. IGF2BP3 knockdown by RNA interference decreases levels of IGF-II protein without affecting IGF-II, c-Myc, or β Actin mRNA and H19 RNA levels. IGF2BP3 is a marker for carcinomas and high-grade dysplastic lesions of pancreatic ductal epithelium.

Background References

1. Davidson, B. et al. VICKZ2 protein expression in ovarian serous carcinoma effusions is associated with poor survival. Human pathology 45:1520-8 (2014).

2. Wang, L. et al. IMP3 is a novel biomarker to predict metastasis and prognosis of gastric adenocarcinoma: a retrospective study. Chin Med J 123:3554-3558 (2010).

Sequence Similarity

Belongs to the RRM IMP/VICKZ family.

Tissue Specificity

Expressed in fetal liver, fetal lung, fetal kidney, fetal thymus, fetal placenta, fetal follicles of ovary and gonocytes of testis, growing oocytes, spermatogonia and semen (at protein level). Expressed in cervix adenocarcinoma, in testicular, pancreatic and renal-cell carcinomas (at protein level). Expressed ubiquitously during fetal development at 8 and 14 weeks of gestation. Expressed in ovary, testis, brain, placenta, pancreatic cancer tissues and pancreatic cancer cell lines.

Subcellular Location

Cytoplasm. Nucleus.

UNIPROT

SwissProt: O00425 Human

SwissProt: Q9CPN8 Mouse

Entrez Gene: 315697 Rat

Synonyms

Cancer/testis antigen 98 antibody

CT98 antibody

DKFZp686F1078 antibody

hKOC antibody

IF2B3_HUMAN antibody

IGF II mRNA binding protein 3 antibody

IGF-II mRNA-binding protein 3 antibody

IGF2 mRNA binding protein 3 antibody

IGF2 mRNA-binding protein 3 antibody

IGF2BP3 antibody

Expand

Images

  • Western blot analysis of IMP3 on different lysates with Mouse anti-IMP3 antibody (<a href="/products/EM1701-15" style="font-weight: bold;text-decoration: underline;">EM1701-15</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: 293T cell lysate (20 µg/Lane)<br />Lane 3: Mouse placenta tissue lysate (40 µg/Lane)<br />Lane 4: Rat placenta tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 64 kDa<br />Observed band size: 64 kDa<br /><br />Exposure time: 12 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM1701-15" style="font-weight: bold;text-decoration: underline;">EM1701-15</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of IMP3 on different lysates with Mouse anti-IMP3 antibody (EM1701-15) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: 293T cell lysate (20 µg/Lane)
    Lane 3: Mouse placenta tissue lysate (40 µg/Lane)
    Lane 4: Rat placenta tissue lysate (40 µg/Lane)

    Predicted band size: 64 kDa
    Observed band size: 64 kDa

    Exposure time: 12 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-15) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of IMP3 on different lysates with Mouse anti-IMP3 antibody (<a href="/products/EM1701-15" style="font-weight: bold;text-decoration: underline;">EM1701-15</a>) at 1/5,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-IMP3 KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 64 kDa<br />Observed band size: 64 kDa<br /><br />Exposure time: 6 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM1701-15" style="font-weight: bold;text-decoration: underline;">EM1701-15</a>) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of IMP3 on different lysates with Mouse anti-IMP3 antibody (EM1701-15) at 1/5,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-IMP3 KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 64 kDa
    Observed band size: 64 kDa

    Exposure time: 6 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-15) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • IMP3 was immunoprecipitated from 0.2 mg 293T cell lysate with <a href="/products/EM1701-15" style="font-weight: bold;text-decoration: underline;">EM1701-15</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/EM1701-15" style="font-weight: bold;text-decoration: underline;">EM1701-15</a> at 1/2,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (<a href="/products/NBI02H" style="font-weight: bold;text-decoration: underline;">NBI02H</a>) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: 293T cell lysate (input)<br />Lane 2: <a href="/products/EM1701-15" style="font-weight: bold;text-decoration: underline;">EM1701-15</a> IP in 293T cell lysate<br />Lane 3: Mouse IgG instead of <a href="/products/EM1701-15" style="font-weight: bold;text-decoration: underline;">EM1701-15</a> in 293T cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 43 seconds

    IMP3 was immunoprecipitated from 0.2 mg 293T cell lysate with EM1701-15 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using EM1701-15 at 1/2,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: 293T cell lysate (input)
    Lane 2: EM1701-15 IP in 293T cell lysate
    Lane 3: Mouse IgG instead of EM1701-15 in 293T cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 43 seconds

  • ICC staining IMP3 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining IMP3 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining IMP3 (green) in SH-SY5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining IMP3 (green) in SH-SY5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-IMP3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-IMP3 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-IMP3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-IMP3 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-IMP3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-IMP3 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-IMP3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-IMP3 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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