CD147 Rabbit Polyclonal Antibody
Catalog# 1003-10
CD147 Rabbit Polyclonal Antibody
-
WB
-
IF-Cell
-
IHC-P
-
FC
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
CD147 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide corresponding to mouse CD147 aa 121-170 / 389 mouse.
Species Reactivity
Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P, FC
Target Molecular Weight
Predicted band size: 42 kDa
Positive Control
Mouse embryo, PC-12 cell lysates, NIH/3T3, F9.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:500
-
IF-Cell
-
1:50
-
FC
-
1ug/mL
Target
Function
CD147 (Basigin) is a member of the immunoglobulin superfamily, with a structure related to the putative primordial form of the family. CD147 is a widely expressed membrane glycoprotein that plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. It stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS) and may target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. In vitro, CD147 promotes outgrowth of astrocytic processes.
Background References
1. "Retina-specific expression of 5A11/Basigin-2, a member of the immunoglobulin gene superfamily."Ochrietor J.D., Moroz T.P., van Ekeris L., Clamp M.F., Jefferson S.C., deCarvalho A.C., Fadool J.M., Wistow G., Muramatsu T., Linser P.J.Invest. Ophthalmol. Vis. Sci. 44:4086-4096(2003)
2. "Basigin expression and regulation in mouse ovary during the sexual maturation and development of corpus luteum."Chang H., Ni H., Ma X.-H., Xu L.-B., Kadomatsu K., Muramatsu T., Yang Z.-M.Mol. Reprod. Dev. 68:135-141(2004)
Tissue Specificity
Isoform 1 is specifically expressed in retina. Isoform 2 is widely expressed, including adult organs, embryos and EC cells. Expressed in spermatozoa.
Post-translational Modification
N-glycosylated. During spermatogenesis, probably deglycosylated during epididymal transit.
Subcellular Location
Cell membrane, Photoreceptor inner segment, Cell projection, cilium, photoreceptor outer segment; Endoplasmic reticulum membrane, Basolateral cell membrane.
Synonyms
5A11 antigen antibody
5F7 antibody
BASI_HUMAN antibody
Basigin (Ok blood group) antibody
Basigin antibody
Blood brain barrier HT7 antigen antibody
Bsg antibody
CD 147 antibody
CD147 antibody
CD147 antigen antibody
ExpandImages
-
Western blot analysis on mouse embryo using anti-CD147 polyclonal antibody.
-
Western blot analysis of CD147 on PC-12 cell lysates with Rabbit anti-CD147 antibody (1003-10) at 1/1,000 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 60 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (1003-10) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of NIH/3T3 cells labeling CD147 with Rabbit anti-CD147 antibody (1003-10) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD147 antibody (1003-10) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. -
Flow cytometric analysis of F9 cells labeling CD147.
Cells were fixed and permeabilized. Then stained with the primary antibody (1003-10, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
