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CD147 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# 1003-10

CD147 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

CD147 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide corresponding to mouse CD147 aa 121-170 / 389 mouse.

Species Reactivity

Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P, FC

Molecular Weight

Predicted band size: 42 kDa

Positive Control

Mouse embryo, PC-12 cell lysates, NIH/3T3, F9.

Conjugation

unconjugated

RRID

AB_3068719

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500

  • IF-Cell

  • 1:50

  • FC

  • 1ug/mL

Target

Function

CD147 (Basigin) is a member of the immunoglobulin superfamily, with a structure related to the putative primordial form of the family. CD147 is a widely expressed membrane glycoprotein that plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. It stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS) and may target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. In vitro, CD147 promotes outgrowth of astrocytic processes.

Background References

1. "Retina-specific expression of 5A11/Basigin-2, a member of the immunoglobulin gene superfamily."Ochrietor J.D., Moroz T.P., van Ekeris L., Clamp M.F., Jefferson S.C., deCarvalho A.C., Fadool J.M., Wistow G., Muramatsu T., Linser P.J.Invest. Ophthalmol. Vis. Sci. 44:4086-4096(2003)

2. "Basigin expression and regulation in mouse ovary during the sexual maturation and development of corpus luteum."Chang H., Ni H., Ma X.-H., Xu L.-B., Kadomatsu K., Muramatsu T., Yang Z.-M.Mol. Reprod. Dev. 68:135-141(2004)

Tissue Specificity

Isoform 1 is specifically expressed in retina. Isoform 2 is widely expressed, including adult organs, embryos and EC cells. Expressed in spermatozoa.

Post-translational Modification

N-glycosylated. During spermatogenesis, probably deglycosylated during epididymal transit.

Subcellular Location

Cell membrane, Photoreceptor inner segment, Cell projection, cilium, photoreceptor outer segment; Endoplasmic reticulum membrane, Basolateral cell membrane.

UNIPROT

SwissProt: P18572 Mouse

SwissProt: P26453 Rat

Synonyms

5A11 antigen antibody

5F7 antibody

BASI_HUMAN antibody

Basigin (Ok blood group) antibody

Basigin antibody

Blood brain barrier HT7 antigen antibody

Bsg antibody

CD 147 antibody

CD147 antibody

CD147 antigen antibody

Expand

Images

  • Western blot analysis on mouse embryo using anti-CD147 polyclonal antibody.

    Western blot analysis on mouse embryo using anti-CD147 polyclonal antibody.

  • Western blot analysis of CD147 on PC-12 cell lysates with Rabbit anti-CD147 antibody (<a href="/products/1003-10" style="font-weight: bold;text-decoration: underline;">1003-10</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 42 kDa<br />Observed band size: 60 kDa<br /><br />Exposure time: 2 minutes;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/1003-10" style="font-weight: bold;text-decoration: underline;">1003-10</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CD147 on PC-12 cell lysates with Rabbit anti-CD147 antibody (1003-10) at 1/1,000 dilution.

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 42 kDa
    Observed band size: 60 kDa

    Exposure time: 2 minutes;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (1003-10) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of NIH/3T3 cells labeling CD147 with Rabbit anti-CD147 antibody (<a href="/products/1003-10" style="font-weight: bold;text-decoration: underline;">1003-10</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD147 antibody (<a href="/products/1003-10" style="font-weight: bold;text-decoration: underline;">1003-10</a>) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of NIH/3T3 cells labeling CD147 with Rabbit anti-CD147 antibody (1003-10) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD147 antibody (1003-10) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  • Flow cytometric analysis of F9 cells labeling CD147.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/1003-10" style="font-weight: bold;text-decoration: underline;">1003-10</a>, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of F9 cells labeling CD147.

    Cells were fixed and permeabilized. Then stained with the primary antibody (1003-10, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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