PRODUCT CODE: ER130905

Alpha-tubulin Antibody (ER130905)

  • Zebrafish

Applications

  • WB

  • IHC-P

  • ICC

  • FC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

Western blot analysis of Alpha-tubulin on different cell lysates using anti-Alpha -tubulin antibody at 1/10000 dilution.<br />
Positive control: <br />
Lane 1: NIH/3T3 <br />
Lane 2: HepG2 <br />
Lane 3: PC12 <br />
Lane 4: Hela
  • Western blot analysis of Alpha-tubulin on different cell lysates using anti-Alpha -tubulin antibody at 1/10000 dilution.<br />
Positive control: <br />
Lane 1: NIH/3T3 <br />
Lane 2: HepG2 <br />
Lane 3: PC12 <br />
Lane 4: Hela
  • ICC staining of Alpha-tubulin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining of Alpha-tubulin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • IHC-P image of Alpha-tubulin staining with Alpha-tubulin antibody on tissue sections from human stomach. The secondary used was an Alexa-Fluor 555 conjugated goat anti-rabbit polyclonal.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Alpha-tubulin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Alpha-tubulin antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with Alpha-tubulin antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
Western blot analysis of Alpha-tubulin on different cell lysates using anti-Alpha -tubulin antibody at 1/10000 dilution.
Positive control:
Lane 1: NIH/3T3
Lane 2: HepG2
Lane 3: PC12
Lane 4: Hela

Applications

  • WB

  • IHC-P

  • ICC

  • FC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Alpha-tubulin Antibody (ER130905)

Immunogen

Peptide

Host

Rabbit

Positive Control

NIH/3T3, Hela, HepG2, PC12, human stomach tissue, human tonsil tissue, mouse pancreas tissue

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

50 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:5,000-1:10,000

  • ICC

  • 1:200-1:500

  • IHC-P

  • 1:200-1:500

  • IF

  • 1:200-1:500

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Tubulin alpha-4A chain

GENE NAME

TUBA4A

SYNONYMS

TBA4A

SEQUENCE SIMILARITIES

Belongs to the tubulin family.

POST-TRANSLATIONAL MODIFICATION

Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold.; Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).; Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly.; Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability.

SUBCELLULAR LOCATION

Cytoplasm, cytoskeleton.

FUNCTION

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

CITATIONS

  • Wang, Yuan-Li et al.

    The E3 Ubiquitin Ligase CRL4 Regulates Proliferation and Progression Through Meiosis in Chinese Mitten Crab Eriocheir sinensis. | Biology of Reproduction [2016]