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PRODUCT CODE: ET1702-05

67kDa Laminin Receptor Recombinant Rabbit Monoclonal Antibody [JF0955] (ET1702-05)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of RPSA on different lysates using anti-RPSA antibody at 1/1,000 dilution.<br /> Positive control: <br /> Lane 1: K562 <br /> Lane 2: HepG2 <br /> Lane 3: A431
  • Western blot analysis of RPSA on different lysates using anti-RPSA antibody at 1/1,000 dilution.<br /> Positive control: <br /> Lane 1: K562 <br /> Lane 2: HepG2 <br /> Lane 3: A431
  • ICC staining RPSA in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining RPSA in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining RPSA in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining RPSA in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-RPSA antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-RPSA antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-RPSA antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of MCF-7 cells with RPSA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of RPSA on different lysates using anti-RPSA antibody at 1/1,000 dilution.
Positive control:
Lane 1: K562
Lane 2: HepG2
Lane 3: A431

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

67kDa Laminin Receptor Recombinant Rabbit Monoclonal Antibody [JF0955] (ET1702-05)

Immunogen

Synthetic peptide within human 67kda laminin receptor aa250-295 / 295.

Host

Rabbit

Positive Control

K562, A431, RH-35, MCF-7, Hela, HepG2, human gastric carcinoma tissue, mouse kidney tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF0955

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

40 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

P08865

PROTEIN NAME

67kDa Laminin Receptor

SYNONYMS

34/67 kDa laminin receptor antibody; 37 kDa laminin receptor precursor antibody; 37/67 kDa laminin receptor antibody; 37LRP antibody; 40S ribosomal protein SA antibody; 67 kDa laminin receptor antibody; 67LR antibody; Colon carcinoma laminin binding protein antibody; Colon carcinoma laminin-binding protein antibody; LAMBR antibody; Laminin receptor 1 antibody; Laminin-binding protein precursor p40 antibody; LAMR 1 antibody; LamR antibody; LAMR1 antibody; LBP antibody; LBP/p40 antibody; LRP antibody; LRP/LR antibody; Multidrug resistance associated protein MGr1 Ag antibody; Multidrug resistance associated protein MGr1Ag antibody; Multidrug resistance-associated protein MGr1-Ag antibody; NEM/1CHD4 antibody; p40 antibody; Ribosomal Protein SA antibody; rpsA antibody; RSSA_HUMAN antibody; SA antibody

SEQUENCE SIMILARITIES

Belongs to the universal ribosomal protein uS2 family.

POST-TRANSLATIONAL MODIFICATION

Acylated. Acylation may be a prerequisite for conversion of the monomeric 37 kDa laminin receptor precursor (37LRP) to the mature dimeric 67 kDa laminin receptor (67LR), and may provide a mechanism for membrane association.; Cleaved by stromelysin-3 (ST3) at the cell surface. Cleavage by stromelysin-3 may be a mechanism to alter cell-extracellular matrix interactions.

SUBCELLULAR LOCATION

Cell membrane, Nucleus, Cytoplasm.

FUNCTION

Laminin receptor (Laminin-R) has a heterodimeric structure similar to that of receptors for other extracellular matrix proteins such as Fibronectin and Vitronectin. Incorporation of Laminin-R into lysosomal membranes makes it possible for lysosomes to attach to surfaces coated with Laminin. This and other properties identify Laminin-R as a member of the integrin family of cell adhesion receptors. The Laminin-R precursor is a polypeptide whose expression is consistently upregulated in aggressive carcinoma. The precursor, which is also identified as p40 ribosome-associated protein, appears to be a multifunctional protein involved in the translational machinery. Laminin-R (also known as colon carcinoma laminin-binding protein) and is found at nine-fold higher levels in colon carcinoma than in adjacent normal colonic epithelium. Additionally, the level of the Laminin-R is higher in the lung cancer cell line than in the lung cell line.