Positive control:
Lane 1: K562
Lane 2: HepG2
Lane 3: A431
Applications
-
WB
-
ICC
-
IF
-
IHC-P
-
IP
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
67kDa Laminin Receptor Recombinant Rabbit Monoclonal Antibody [JF0955] (ET1702-05)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
K562, A431, RH-35, MCF-7, Hela, HepG2, human gastric carcinoma tissue, mouse kidney tissue, mouse brain tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JF0955
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A purified.
MOLECULAR WEIGHT
40 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:2,000
-
ICC/IF
-
1:100-1:500
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
67kDa Laminin Receptor
SYNONYMS
34/67 kDa laminin receptor antibody; 37 kDa laminin receptor precursor antibody; 37/67 kDa laminin receptor antibody; 37LRP antibody; 40S ribosomal protein SA antibody; 67 kDa laminin receptor antibody; 67LR antibody; Colon carcinoma laminin binding protein antibody; Colon carcinoma laminin-binding protein antibody; LAMBR antibody; Laminin receptor 1 antibody; Laminin-binding protein precursor p40 antibody; LAMR 1 antibody; LamR antibody; LAMR1 antibody; LBP antibody; LBP/p40 antibody; LRP antibody; LRP/LR antibody; Multidrug resistance associated protein MGr1 Ag antibody; Multidrug resistance associated protein MGr1Ag antibody; Multidrug resistance-associated protein MGr1-Ag antibody; NEM/1CHD4 antibody; p40 antibody; Ribosomal Protein SA antibody; rpsA antibody; RSSA_HUMAN antibody; SA antibody
SEQUENCE SIMILARITIES
Belongs to the universal ribosomal protein uS2 family.
POST-TRANSLATIONAL MODIFICATION
Acylated. Acylation may be a prerequisite for conversion of the monomeric 37 kDa laminin receptor precursor (37LRP) to the mature dimeric 67 kDa laminin receptor (67LR), and may provide a mechanism for membrane association.; Cleaved by stromelysin-3 (ST3) at the cell surface. Cleavage by stromelysin-3 may be a mechanism to alter cell-extracellular matrix interactions.
SUBCELLULAR LOCATION
Cell membrane, Nucleus, Cytoplasm.
FUNCTION
Laminin receptor (Laminin-R) has a heterodimeric structure similar to that of receptors for other extracellular matrix proteins such as Fibronectin and Vitronectin. Incorporation of Laminin-R into lysosomal membranes makes it possible for lysosomes to attach to surfaces coated with Laminin. This and other properties identify Laminin-R as a member of the integrin family of cell adhesion receptors. The Laminin-R precursor is a polypeptide whose expression is consistently upregulated in aggressive carcinoma. The precursor, which is also identified as p40 ribosome-associated protein, appears to be a multifunctional protein involved in the translational machinery. Laminin-R (also known as colon carcinoma laminin-binding protein) and is found at nine-fold higher levels in colon carcinoma than in adjacent normal colonic epithelium. Additionally, the level of the Laminin-R is higher in the lung cancer cell line than in the lung cell line.