PRODUCT CODE: ET1702-80

58K Golgi protein Recombinant Rabbit Monoclonal Antibody [JF099-5] (ET1702-80)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

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Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-58K Golgi protein antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-58K Golgi protein antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-58K Golgi protein antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with 58K Golgi protein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-58K Golgi protein antibody. Counter stained with hematoxylin.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

58K Golgi protein Recombinant Rabbit Monoclonal Antibody [JF099-5] (ET1702-80)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human liver tissue, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF099-5

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

59 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • FC

  • 1:50-1:100

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

58K Golgi protein

SYNONYMS

Formimidoyltetrahydrofolate cyclodeaminase antibody; Formimidoyltransferase cyclodeaminase antibody; Formiminotetrahydrofolate cyclodeaminase antibody; Formiminotransferase cyclodeaminase antibody; Formiminotransferase-cyclodeaminase antibody; FTCD antibody; FTCD_HUMAN antibody; Glutamate formiminotransferase antibody; Glutamate formyltransferase antibody; LCHC 1 antibody; LCHC1 antibody

SEQUENCE SIMILARITIES

In the C-terminal section; belongs to the cyclodeaminase/cyclohydrolase family.; In the N-terminal section; belongs to the formiminotransferase family.

SUBCELLULAR LOCATION

Cytoplasm, Golgi apparatus.

FUNCTION

58K protein antibodies are excellent for use as markers for the Golgi complex. The 58K protein has been identified as being FTCD, a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Defects in FTCD are the cause of glutamate formiminotransferase deficiency [also known as formiminoglutamicaciduria (FIGLU-uria)], an autosomal recessive disorder. Features of a severe phenotype include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay and no hematological abnormalities.