2B4 Rabbit Polyclonal Antibody (HA500180)
Catalog# HA500180
Rabbit Polyclonal to 2B4
Tested for
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IHC-P
Reacts with
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Human
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-2B4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500180, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.