PRODUCT CODE: ET1701-1

14-3-3 epsilon Recombinant Rabbit Monoclonal Antibody [JJ08-40] (ET1701-1)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of 14-3-3 epsilon on different lysates using anti-14-3-3 epsilon antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: SH-SY-5Y         <br />
Lane 2: 293T
  • Western blot analysis of 14-3-3 epsilon on different lysates using anti-14-3-3 epsilon antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: SH-SY-5Y         <br />
Lane 2: 293T
  • ICC staining 14-3-3 epsilon in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining 14-3-3 epsilon in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining 14-3-3 epsilon in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-14-3-3 epsilon antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-14-3-3 epsilon antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY-5Y cells with 14-3-3 epsilon antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of 14-3-3 epsilon on different lysates using anti-14-3-3 epsilon antibody at 1/1,000 dilution.
Positive control:
Lane 1: SH-SY-5Y
Lane 2: 293T

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

14-3-3 epsilon Recombinant Rabbit Monoclonal Antibody [JJ08-40] (ET1701-1)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

SH-SY-5Y, NIH/3T3, Hela, 293T, human colon cancer tissue, mouse placenta tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ08-40

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

29 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:10-1:100

TARGET

UNIPROT #

PROTEIN NAME

14-3-3 epsilon

SYNONYMS

14 3 3 E antibody; 14 3 3 epsilon antibody; 14 3 3E antibody; 14-3-3 protein epsilon antibody; 14-3-3E antibody; 1433E_HUMAN antibody; Epididymis luminal protein 2 antibody; FLJ45465 antibody; FLJ53559 antibody; HEL2 antibody; KCIP 1 antibody; KCIP1 antibody; MDCR antibody; MDS antibody; Mitochondrial import stimulation factor L subunit antibody; Protein kinase C inhibitor protein1 antibody; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, epsilon antibody; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, epsilon polypeptide antibody; Tyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide antibody; YWHAE antibody

SEQUENCE SIMILARITIES

Belongs to the 14-3-3 family.

SUBCELLULAR LOCATION

Cytoplasm, Melanosome.

FUNCTION

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ and η that have been identified in mammals. The 14-3-3 epsilon, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer.