PRODUCT CODE: 0407-5

14-3-3 beta/alpha Rabbit Polyclonal Antibody (0407-5)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis on different cell and tissue using anti-14-3-3 b/a polyclonal antibody.
  • Western blot analysis on different cell and tissue using anti-14-3-3 b/a polyclonal antibody.
  • ICC staining 14-3-3 b/a in N2A cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis on different cell and tissue using anti-14-3-3 b/a polyclonal antibody.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

14-3-3 beta/alpha Rabbit Polyclonal Antibody (0407-5)

Immunogen

Synthetic peptide within mouse 14-3-3 beta / alpha aa 197-246 / 246.

Host

Rabbit

Positive Control

Hela, human brain, mouse brain, N2A

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

MOLECULAR WEIGHT

28 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:5,000

  • ICC

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

14-3-3 beta/alpha

SYNONYMS

14 3 3 alpha antibody; 14 3 3 protein beta/alpha antibody; 14-3-3 protein beta/alpha antibody; 1433B_HUMAN antibody; Brain protein 14 3 3 beta isoform antibody; GW128 antibody; HS 1 antibody; KCIP-1 antibody; KCIP1 antibody; N-terminally processed antibody; Protein 1054 antibody; Protein kinase C inhibitor protein 1 antibody; YWHAA antibody; YWHAB antibody

SEQUENCE SIMILARITIES

Belongs to the 14-3-3 family.

POST-TRANSLATIONAL MODIFICATION

Isoform alpha differs from isoform beta in being phosphorylated (By similarity). Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.; Isoform Short contains a N-acetylmethionine at position 1.

SUBCELLULAR LOCATION

Cytoplasm Melanosome

FUNCTION

The 14-3-3 proteins are a family of proteins involved in the regulation of apoptosis, mitogenic signaling and cell-cycle checkpoints. The 14-3-3 proteins are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins. Through binding Bad, 14-3-3 prevents apoptosis by sequestering Bad to the cytosol. The 14-3-3 proteins are also Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. They bind to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.