PRODUCT CODE: M0407-14

14-3-3 b/a Mouse Monoclonal Antibody [9-26] (M0407-14)

  • Zebrafish

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

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Western blot analysis on mouse liver lysates using anti-14-3-3b/a Mouse mAb (Cat. # M0407-14).
  • Western blot analysis on mouse liver lysates using anti-14-3-3b/a Mouse mAb (Cat. # M0407-14).
  • Immunofluorescent staining of Hela cells using anti-14-3-3b/a Mouse mAb (Cat. # M0407-14).
  • Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using anti-14-3-3b/a Mouse mAb (Cat. # M0407-14).
  • Western blot analysis of 14-3-3b/a on hybrid fish (crucian-carp) brain tissue lysate using anti-14-3-3b/a antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded Zebrafish tissue using anti-14-3-3b/a antibody. Counter stained with hematoxylin.
Western blot analysis on mouse liver lysates using anti-14-3-3b/a Mouse mAb (Cat. # M0407-14).

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

14-3-3 b/a Mouse Monoclonal Antibody [9-26] (M0407-14)

Immunogen

Peptide

Host

Mouse

Positive Control

mouse liver Hela

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

9-26

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

28 kDa

Isotype

IgG2a

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC

  • 1:100

  • IHC-P

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

14-3-3 b/a

SYNONYMS

14 3 3 alpha antibody; 14 3 3 protein beta/alpha antibody; 14-3-3 protein beta/alpha antibody; 1433B_HUMAN antibody; Brain protein 14 3 3 beta isoform antibody; GW128 antibody; HS 1 antibody; KCIP-1 antibody; KCIP1 antibody; N-terminally processed antibody; Protein 1054 antibody; Protein kinase C inhibitor protein 1 antibody; YWHAA antibody; YWHAB antibody

SEQUENCE SIMILARITIES

Belongs to the 14-3-3 family.

POST-TRANSLATIONAL MODIFICATION

The alpha, brain-specific form differs from the beta form in being phosphorylated. Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.

SUBCELLULAR LOCATION

Cytoplasm

FUNCTION

The 14-3-3 proteins are a family of proteins involved in the regulation of apoptosis, mitogenic signaling and cell-cycle checkpoints. The 14-3-3 proteins are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins. Through binding Bad, 14-3-3 prevents apoptosis by sequestering Bad to the cytosol. The 14-3-3 proteins are also Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. They bind to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.