14-3-3 alpha+beta Recombinant Rabbit Monoclonal Antibody [SD0837] (ET1612-99)
Catalog# ET1612-99
Rabbit Monoclonal to 14-3-3 alpha+beta
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WB
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IF-Cell
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IHC-P
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FC
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IP
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Human
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Mouse
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Rat
Lane 1: Neuro-2a cell lysate (20 µg/Lane)
Lane 2: C6 cell lysate (20 µg/Lane)
Lane 3: Mouse brain tissue lysate (40 µg/Lane)
Lane 4: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 14 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-99) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
14-3-3 alpha+beta Recombinant Rabbit Monoclonal Antibody [SD0837] (ET1612-99)
Immunogen
Synthetic peptide corresponding to c terminal human 14-3-3 alpha + beta aa 197-246 / 246.
Host
Rabbit
Positive Control
Neuro-2a cell lysate, C6 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, Hela cell lysate, HepG2 cell lysate, 293T cell lysate, SK-Br-3 cell lysate, Hela, A431, Neuro-2a , human breast carcinoma tissue, mouse brain tissue, mouse skin tissue, human breast tissue, rat brain tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SD0837
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IF-Cell
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1:100-1:500
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IHC-P
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1:50-1:200
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FC
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1:1,000
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IP
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Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 28 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
14-3-3 alpha+beta
SYNONYMS
14 3 3 alpha antibody;14 3 3 protein beta/alpha antibody;14-3-3 protein beta/alpha antibody;1433B_HUMAN antibody;Brain protein 14 3 3 beta isoform antibody;GW128 antibody;HS 1 antibody;KCIP-1 antibody;KCIP1 antibody;N-terminally processed antibody;Protein 1054 antibody;Protein kinase C inhibitor protein 1 antibody;YWHAA antibody;YWHAB antibody
SEQUENCE SIMILARITIES
Belongs to the 14-3-3 family.
POST-TRANSLATIONAL MODIFICATION
The alpha, brain-specific form differs from the beta form in being phosphorylated. Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.
SUBCELLULAR LOCATION
Cytoplasm, Melanosome.
FUNCTION
14-3-3 protein beta/alpha is a protein that in humans is encoded by the YWHAB gene. This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene.
CITATIONS
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The role of 14-3-3 in the progression of vascular inflammation induced by lipopolysaccharide
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WB
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Human
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Citation
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